Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9438 | 3' | -50.5 | NC_002531.1 | + | 63448 | 0.66 | 0.991922 |
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Target: 5'- --cCAUUCCUGagGCCCuCAGgcACGgCACUg -3' miRNA: 3'- agaGUAAGGAU--UGGG-GUU--UGCgGUGA- -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 121796 | 0.66 | 0.990714 |
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Target: 5'- -aUCAgagCCgucagGGCCCCAAcagcCGCCACc -3' miRNA: 3'- agAGUaa-GGa----UUGGGGUUu---GCGGUGa -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 32476 | 0.66 | 0.990714 |
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Target: 5'- gCUCAgcagcUCaCUAgcuGCUgCGAGCGCCACUc -3' miRNA: 3'- aGAGUa----AG-GAU---UGGgGUUUGCGGUGA- -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 74587 | 0.66 | 0.986227 |
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Target: 5'- cCUCAggCCUucgAGCCUUuAGCGCCAUc -3' miRNA: 3'- aGAGUaaGGA---UUGGGGuUUGCGGUGa -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 15728 | 0.66 | 0.986227 |
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Target: 5'- gCUCAgagcaCUUAACCCCAGAauCGCUggGCUg -3' miRNA: 3'- aGAGUaa---GGAUUGGGGUUU--GCGG--UGA- -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 69189 | 0.67 | 0.980239 |
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Target: 5'- gCUCug-Caac-CCCCAGACGCUACUa -3' miRNA: 3'- aGAGuaaGgauuGGGGUUUGCGGUGA- -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 78214 | 0.67 | 0.977865 |
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Target: 5'- gUCUCcaugUCCUGuCCCCAcaucacaGCCACa -3' miRNA: 3'- -AGAGua--AGGAUuGGGGUuug----CGGUGa -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 24899 | 0.68 | 0.966246 |
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Target: 5'- aUCUUc-UCCUGGCCUC--ACGCCACc -3' miRNA: 3'- -AGAGuaAGGAUUGGGGuuUGCGGUGa -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 10296 | 0.69 | 0.955094 |
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Target: 5'- uUCUCAgaaCUAACCU---ACGCCACa -3' miRNA: 3'- -AGAGUaagGAUUGGGguuUGCGGUGa -5' |
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| 9438 | 3' | -50.5 | NC_002531.1 | + | 60220 | 1.1 | 0.007127 |
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Target: 5'- gUCUCAUUCCUAACCCCAAACGCCACUg -3' miRNA: 3'- -AGAGUAAGGAUUGGGGUUUGCGGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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