Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9440 | 5' | -54.1 | NC_002531.1 | + | 122421 | 0.66 | 0.903186 |
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Target: 5'- aUAGGCAGAGCucCGCCa--CGCAGg- -3' miRNA: 3'- cAUUCGUUUCGu-GCGGacgGCGUCac -5' |
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| 9440 | 5' | -54.1 | NC_002531.1 | + | 46976 | 0.67 | 0.874936 |
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Target: 5'- -aGAGuCAAcAGCcccaGCGCCUGCCuGCAGg- -3' miRNA: 3'- caUUC-GUU-UCG----UGCGGACGG-CGUCac -5' |
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| 9440 | 5' | -54.1 | NC_002531.1 | + | 126727 | 0.69 | 0.759565 |
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Target: 5'- ---uGCAAGGCugGCUgucgaUGCUGCAGa- -3' miRNA: 3'- cauuCGUUUCGugCGG-----ACGGCGUCac -5' |
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| 9440 | 5' | -54.1 | NC_002531.1 | + | 128121 | 0.7 | 0.708496 |
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Target: 5'- aGUAAGCAGcauGCAUGCCUGCaaaaGUuuaaAGUGc -3' miRNA: 3'- -CAUUCGUUu--CGUGCGGACGg---CG----UCAC- -5' |
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| 9440 | 5' | -54.1 | NC_002531.1 | + | 54406 | 0.74 | 0.488269 |
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Target: 5'- -aGGGCGGuucGGCGCGCCUGCCGguuagcgGGUGg -3' miRNA: 3'- caUUCGUU---UCGUGCGGACGGCg------UCAC- -5' |
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| 9440 | 5' | -54.1 | NC_002531.1 | + | 63010 | 1.09 | 0.002849 |
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Target: 5'- uGUAAGCAAAGCACGCCUGCCGCAGUGg -3' miRNA: 3'- -CAUUCGUUUCGUGCGGACGGCGUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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