Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9442 | 5' | -56.4 | NC_002531.1 | + | 9881 | 0.66 | 0.835294 |
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Target: 5'- uGUGAAG-CUGCCUUUGGCCUUuaGGu-- -3' miRNA: 3'- -CACUUCuGAUGGGGACCGGGA--CCuua -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 124386 | 0.66 | 0.817963 |
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Target: 5'- -gGAAaACUcACCCCUGGCCucuugcCUGGGGg -3' miRNA: 3'- caCUUcUGA-UGGGGACCGG------GACCUUa -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 118081 | 0.68 | 0.722145 |
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Target: 5'- aUGAAGACggggACCCCgagGGaCCagaUGGAAc -3' miRNA: 3'- cACUUCUGa---UGGGGa--CC-GGg--ACCUUa -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 118150 | 0.68 | 0.722145 |
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Target: 5'- aUGAAGACggggACCCCgagGGaCCagaUGGAAc -3' miRNA: 3'- cACUUCUGa---UGGGGa--CC-GGg--ACCUUa -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 116889 | 0.68 | 0.691363 |
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Target: 5'- uUGggGACUuagaguuggagGCCCCUGGCuuCCcGGAc- -3' miRNA: 3'- cACuuCUGA-----------UGGGGACCG--GGaCCUua -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 102111 | 0.69 | 0.680986 |
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Target: 5'- --aGGGACUGCCaaaCUGGCCUaUGGAGa -3' miRNA: 3'- cacUUCUGAUGGg--GACCGGG-ACCUUa -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 62137 | 0.7 | 0.59721 |
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Target: 5'- gGUGAAGGCUaaaaagcauGCUCCUGGCCUuaUGGc-- -3' miRNA: 3'- -CACUUCUGA---------UGGGGACCGGG--ACCuua -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 118240 | 0.71 | 0.51507 |
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Target: 5'- -cGggGACcucuugaGCCCCUGGCCCaucUGGGc- -3' miRNA: 3'- caCuuCUGa------UGGGGACCGGG---ACCUua -5' |
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| 9442 | 5' | -56.4 | NC_002531.1 | + | 69438 | 1.05 | 0.003479 |
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Target: 5'- gGUGAAGACUACCCCUGGCCCUGGAAUu -3' miRNA: 3'- -CACUUCUGAUGGGGACCGGGACCUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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