Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9443 | 3' | -51.6 | NC_002531.1 | + | 20135 | 0.66 | 0.980581 |
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Target: 5'- aGAUagGCUaucgGGUGUUUGa--GGCCAACg -3' miRNA: 3'- aCUA--CGGg---CCACAAACauaCCGGUUG- -5' |
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| 9443 | 3' | -51.6 | NC_002531.1 | + | 34087 | 0.67 | 0.959655 |
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Target: 5'- gUGGUGCuCUGGccgGgg-GUAUGGCCGu- -3' miRNA: 3'- -ACUACG-GGCCa--CaaaCAUACCGGUug -5' |
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| 9443 | 3' | -51.6 | NC_002531.1 | + | 48019 | 0.69 | 0.909428 |
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Target: 5'- gUGAUGCugCCGGUGUgcaccaacgggcauaUGUGUgguaugggGGCCGACu -3' miRNA: 3'- -ACUACG--GGCCACAa--------------ACAUA--------CCGGUUG- -5' |
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| 9443 | 3' | -51.6 | NC_002531.1 | + | 4345 | 0.71 | 0.841067 |
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Target: 5'- cGGUGCauggCGGUGUcacucgUGUAuaguucuacuUGGCCAGCg -3' miRNA: 3'- aCUACGg---GCCACAa-----ACAU----------ACCGGUUG- -5' |
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| 9443 | 3' | -51.6 | NC_002531.1 | + | 103681 | 0.76 | 0.576112 |
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Target: 5'- aUGGUGCCgGGUGUa-GUcAUGGCCAAg -3' miRNA: 3'- -ACUACGGgCCACAaaCA-UACCGGUUg -5' |
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| 9443 | 3' | -51.6 | NC_002531.1 | + | 88863 | 1.11 | 0.004008 |
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Target: 5'- gUGAUGCCCGGUGUUUGUAUGGCCAACg -3' miRNA: 3'- -ACUACGGGCCACAAACAUACCGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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