Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9444 | 3' | -53.4 | NC_002531.1 | + | 15693 | 0.66 | 0.959693 |
Target: 5'- uCCACAGGGuacAGAGUugCuuuuCUGCgGUg -3' miRNA: 3'- -GGUGUCUCu--UCUCGugGu---GAUGgCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 8158 | 0.66 | 0.955869 |
Target: 5'- cCCACAGuGggGuGCAaagcCCACaUugUGCa -3' miRNA: 3'- -GGUGUCuCuuCuCGU----GGUG-AugGCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 87263 | 0.67 | 0.938138 |
Target: 5'- gCUACAGAGAGGAGCcuaaaaaguucACCcccaaGCcgACUGCa -3' miRNA: 3'- -GGUGUCUCUUCUCG-----------UGG-----UGa-UGGCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 46293 | 0.67 | 0.92221 |
Target: 5'- gCGCAGcuugacGGAGAGCACCGuguCUaucagGCCGCg -3' miRNA: 3'- gGUGUCu-----CUUCUCGUGGU---GA-----UGGCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 19026 | 0.67 | 0.916395 |
Target: 5'- aCUAUAGAgcuGAAGAGUgugGCCAC-ACUGCUc -3' miRNA: 3'- -GGUGUCU---CUUCUCG---UGGUGaUGGCGA- -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 62992 | 0.67 | 0.916395 |
Target: 5'- gCCGCAGuGggGGGCguGCCACaguUUGCa -3' miRNA: 3'- -GGUGUCuCuuCUCG--UGGUGau-GGCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 103801 | 0.67 | 0.909084 |
Target: 5'- uCCAgGGGGcccacuucCACCGCUGCCGCUu -3' miRNA: 3'- -GGUgUCUCuucuc---GUGGUGAUGGCGA- -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 34247 | 0.68 | 0.890634 |
Target: 5'- uCCACGGccauacccccGGccAGAGCACCACUAuCCaGCUu -3' miRNA: 3'- -GGUGUC----------UCu-UCUCGUGGUGAU-GG-CGA- -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 288 | 0.68 | 0.883583 |
Target: 5'- gCugGGAGAGGGGCuauCUGCUGCgcggCGCUu -3' miRNA: 3'- gGugUCUCUUCUCGu--GGUGAUG----GCGA- -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 90970 | 0.7 | 0.810235 |
Target: 5'- -gGCAGAGAGGAGCAUggucaGCUcCUGCa -3' miRNA: 3'- ggUGUCUCUUCUCGUGg----UGAuGGCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 45979 | 0.7 | 0.810235 |
Target: 5'- aCCACAucagugcuGAGAuAGAGCuaGCCAUUGCCGaCUu -3' miRNA: 3'- -GGUGU--------CUCU-UCUCG--UGGUGAUGGC-GA- -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 81293 | 0.72 | 0.692302 |
Target: 5'- aCC-CAGAGGaacuagAGAGUACCauGCUGCUGCa -3' miRNA: 3'- -GGuGUCUCU------UCUCGUGG--UGAUGGCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 99617 | 0.73 | 0.660949 |
Target: 5'- aCCAgCAGuucccuuuAGAAGAGCGUCGCUGCUGCg -3' miRNA: 3'- -GGU-GUC--------UCUUCUCGUGGUGAUGGCGa -5' |
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9444 | 3' | -53.4 | NC_002531.1 | + | 91159 | 1.09 | 0.004172 |
Target: 5'- uCCACAGAGAAGAGCACCACUACCGCUc -3' miRNA: 3'- -GGUGUCUCUUCUCGUGGUGAUGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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