Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9447 | 5' | -48.3 | NC_002531.1 | + | 30704 | 0.66 | 0.999121 |
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Target: 5'- --cAGUCAcGAaCCauuGCUCACCAAACCa -3' miRNA: 3'- ccuUCGGUuUUaGG---UGGGUGGUUUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 94844 | 0.66 | 0.999191 |
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Target: 5'- uGAAGCUAAAgacacuguggcauauGguggCC-CCCACaCGAGCCa -3' miRNA: 3'- cCUUCGGUUU---------------Ua---GGuGGGUG-GUUUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 102396 | 0.66 | 0.999272 |
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Target: 5'- aGGAccCCAAAgauaaGUCUacacccaACCCAcCCAGACCu -3' miRNA: 3'- -CCUucGGUUU-----UAGG-------UGGGU-GGUUUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 35326 | 0.66 | 0.999288 |
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Target: 5'- cGAAGCCcAGGUCgGCUggguCACUGGACUa -3' miRNA: 3'- cCUUCGGuUUUAGgUGG----GUGGUUUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 46303 | 0.66 | 0.999288 |
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Target: 5'- cGGAGGCgCuag--CCACUC-CCAGGCa -3' miRNA: 3'- -CCUUCG-GuuuuaGGUGGGuGGUUUGg -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 103711 | 0.66 | 0.999288 |
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Target: 5'- cGGAGCCAGAG-CCugCaGCUGAcCCg -3' miRNA: 3'- cCUUCGGUUUUaGGugGgUGGUUuGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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