Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9447 | 5' | -48.3 | NC_002531.1 | + | 123174 | 0.72 | 0.938808 |
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Target: 5'- uGGaAAGUCAAAGcgacCCACCCcgggcuguugcaccACCAGGCCa -3' miRNA: 3'- -CC-UUCGGUUUUa---GGUGGG--------------UGGUUUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 124021 | 0.66 | 0.998921 |
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Target: 5'- aGGAGGCCAAGugaCACUCGuaAAGCa -3' miRNA: 3'- -CCUUCGGUUUuagGUGGGUggUUUGg -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 124684 | 0.67 | 0.996138 |
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Target: 5'- ---uGCCAAcuUCCACCCGCauuACa -3' miRNA: 3'- ccuuCGGUUuuAGGUGGGUGguuUGg -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 129478 | 0.71 | 0.968738 |
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Target: 5'- aGGuccuuGGgCGGGGUCUugCCugCAGGCCc -3' miRNA: 3'- -CCu----UCgGUUUUAGGugGGugGUUUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 129556 | 0.71 | 0.965424 |
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Target: 5'- cGGGccugcAGCCAAGA---ACCCACCAaaaGACCc -3' miRNA: 3'- -CCU-----UCGGUUUUaggUGGGUGGU---UUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 130002 | 0.71 | 0.971222 |
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Target: 5'- aGGAGGCCuAGGUCCuuacgugcuaccUCCACCuuuACCu -3' miRNA: 3'- -CCUUCGGuUUUAGGu-----------GGGUGGuu-UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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