Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9447 | 5' | -48.3 | NC_002531.1 | + | 35382 | 0.67 | 0.996138 |
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Target: 5'- uGGAGCCGcuAUCUACCCcUCAGcACUu -3' miRNA: 3'- cCUUCGGUuuUAGGUGGGuGGUU-UGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 86406 | 0.67 | 0.996138 |
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Target: 5'- uGGggGUC----UCCACCUAUCuauACCu -3' miRNA: 3'- -CCuuCGGuuuuAGGUGGGUGGuu-UGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 113454 | 0.67 | 0.996138 |
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Target: 5'- uGGcAGCCGcaccagCCACUCGCCGcagacggucAGCCa -3' miRNA: 3'- -CCuUCGGUuuua--GGUGGGUGGU---------UUGG- -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 43319 | 0.67 | 0.996138 |
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Target: 5'- --uGGUUAGAAUCCugCUAuCCAAGCa -3' miRNA: 3'- ccuUCGGUUUUAGGugGGU-GGUUUGg -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 87494 | 0.68 | 0.995461 |
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Target: 5'- uGGAcGCCAgcGAGUCCcucaagcaGCCaGCCGAACa -3' miRNA: 3'- -CCUuCGGU--UUUAGG--------UGGgUGGUUUGg -5' |
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| 9447 | 5' | -48.3 | NC_002531.1 | + | 35326 | 0.66 | 0.999288 |
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Target: 5'- cGAAGCCcAGGUCgGCUggguCACUGGACUa -3' miRNA: 3'- cCUUCGGuUUUAGgUGG----GUGGUUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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