Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9452 | 3' | -56 | NC_002531.1 | + | 116785 | 0.66 | 0.86124 |
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Target: 5'- cGAUGAUCCAGGggagGGGacuucgCAGGGa--- -3' miRNA: 3'- uCUACUAGGUCUa---CCCg-----GUCCCagag -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 34534 | 0.68 | 0.744736 |
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Target: 5'- gAGAUcGUCCuGggGGGCgGGGGgugCUCc -3' miRNA: 3'- -UCUAcUAGGuCuaCCCGgUCCCa--GAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 117073 | 0.69 | 0.724752 |
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Target: 5'- uGAcGAUCUAGAUGGucguccgggaaGCCAGGGgcCUCc -3' miRNA: 3'- uCUaCUAGGUCUACC-----------CGGUCCCa-GAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 119276 | 0.7 | 0.663117 |
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Target: 5'- aGGA-GAUagcCCAGAUGGGCCAGaugCUCa -3' miRNA: 3'- -UCUaCUA---GGUCUACCCGGUCccaGAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 118788 | 0.75 | 0.355066 |
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Target: 5'- gAGAUagcCCAGAUGGGCCAGGuG-CUCa -3' miRNA: 3'- -UCUAcuaGGUCUACCCGGUCC-CaGAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 118597 | 0.75 | 0.355066 |
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Target: 5'- gAGAUagcCCAGAUGGGCCAGGuG-CUCa -3' miRNA: 3'- -UCUAcuaGGUCUACCCGGUCC-CaGAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 119048 | 0.76 | 0.347099 |
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Target: 5'- aGGA-GAUagcCCAGAUGGGCCAGGuG-CUCa -3' miRNA: 3'- -UCUaCUA---GGUCUACCCGGUCC-CaGAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 119591 | 0.76 | 0.347099 |
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Target: 5'- aGGA-GAUagcCCAGAUGGGCCAGGuG-CUCa -3' miRNA: 3'- -UCUaCUA---GGUCUACCCGGUCC-CaGAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 118410 | 0.82 | 0.13493 |
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Target: 5'- aGGA-GAUagcCCAGAUGGGCCAGGGgCUCa -3' miRNA: 3'- -UCUaCUA---GGUCUACCCGGUCCCaGAG- -5' |
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| 9452 | 3' | -56 | NC_002531.1 | + | 119750 | 1.1 | 0.001727 |
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Target: 5'- gAGAUGAUCCAGAUGGGCCAGGGUCUCa -3' miRNA: 3'- -UCUACUAGGUCUACCCGGUCCCAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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