Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9453 | 5' | -52 | NC_002531.1 | + | 41321 | 0.66 | 0.976277 |
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Target: 5'- --cUCCUGAGGAC-CGGGAgacucuguUUUAUGUg -3' miRNA: 3'- aacAGGGCUCUUGaGCCCU--------GAAUACA- -5' |
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| 9453 | 5' | -52 | NC_002531.1 | + | 35330 | 0.66 | 0.97068 |
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Target: 5'- -aGUCCCuGGAcauCUUGGGGCgcUAUGUg -3' miRNA: 3'- aaCAGGGcUCUu--GAGCCCUGa-AUACA- -5' |
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| 9453 | 5' | -52 | NC_002531.1 | + | 120940 | 0.66 | 0.97068 |
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Target: 5'- gUGUCCCGAGuuCUaGGGACcUAcuUGUu -3' miRNA: 3'- aACAGGGCUCuuGAgCCCUGaAU--ACA- -5' |
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| 9453 | 5' | -52 | NC_002531.1 | + | 82350 | 0.7 | 0.835617 |
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Target: 5'- ---cUCCGGGAGCUCGGGGCc-GUGg -3' miRNA: 3'- aacaGGGCUCUUGAGCCCUGaaUACa -5' |
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| 9453 | 5' | -52 | NC_002531.1 | + | 121090 | 0.75 | 0.58162 |
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Target: 5'- -gGUCCCuAGAACUCGGGACa----- -3' miRNA: 3'- aaCAGGGcUCUUGAGCCCUGaauaca -5' |
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| 9453 | 5' | -52 | NC_002531.1 | + | 120913 | 0.78 | 0.439789 |
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Target: 5'- -aGUCCCGAGuuCUCGGGACaagAUGg -3' miRNA: 3'- aaCAGGGCUCuuGAGCCCUGaa-UACa -5' |
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| 9453 | 5' | -52 | NC_002531.1 | + | 121064 | 1.06 | 0.008681 |
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Target: 5'- cUUGUCCCGAGAACUCGGGACUUAUGUa -3' miRNA: 3'- -AACAGGGCUCUUGAGCCCUGAAUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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