Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9454 | 5' | -57.6 | NC_002531.1 | + | 55127 | 0.66 | 0.801899 |
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Target: 5'- uGGCcgGGUcgccauGCUGCCaUGCcGGGUa- -3' miRNA: 3'- uCCGuaCCA------UGACGGcACGaCCCAgg -5' |
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| 9454 | 5' | -57.6 | NC_002531.1 | + | 66616 | 0.66 | 0.791953 |
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Target: 5'- cGGUGUGGUGC--CCGUGCUGaaccaaaGGcCCa -3' miRNA: 3'- uCCGUACCAUGacGGCACGAC-------CCaGG- -5' |
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| 9454 | 5' | -57.6 | NC_002531.1 | + | 28435 | 0.66 | 0.783685 |
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Target: 5'- uAGGCuaaAUGGgggugACUGCUcuaaaaauuaGUGCUGGGcUUCu -3' miRNA: 3'- -UCCG---UACCa----UGACGG----------CACGACCC-AGG- -5' |
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| 9454 | 5' | -57.6 | NC_002531.1 | + | 87681 | 0.67 | 0.773429 |
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Target: 5'- gGGGCAguauuagUGG-ACUGCaCG-GCUGuGGUCa -3' miRNA: 3'- -UCCGU-------ACCaUGACG-GCaCGAC-CCAGg -5' |
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| 9454 | 5' | -57.6 | NC_002531.1 | + | 35702 | 0.67 | 0.73591 |
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Target: 5'- uGGCggGGUAUgUGCUGcguagGCUGGG-CCu -3' miRNA: 3'- uCCGuaCCAUG-ACGGCa----CGACCCaGG- -5' |
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| 9454 | 5' | -57.6 | NC_002531.1 | + | 21731 | 0.69 | 0.614142 |
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Target: 5'- -aGCAUGGUuCUGCCuUGCaGGGUUa -3' miRNA: 3'- ucCGUACCAuGACGGcACGaCCCAGg -5' |
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| 9454 | 5' | -57.6 | NC_002531.1 | + | 9928 | 0.76 | 0.294185 |
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Target: 5'- gAGG-AUGuGUGCUGCCuUGCUGGGUgCa -3' miRNA: 3'- -UCCgUAC-CAUGACGGcACGACCCAgG- -5' |
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| 9454 | 5' | -57.6 | NC_002531.1 | + | 121650 | 1.03 | 0.003875 |
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Target: 5'- gAGGCAUGGUACUGCCGUGCUGGG-CCa -3' miRNA: 3'- -UCCGUACCAUGACGGCACGACCCaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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