miRNA: 3'- -GUG-GGUCCCCCUgUCGGGc--CG---AGAGG- -5'
miRNA: 3'- gUGGGUCCCCc----UGuCGGGCcGAGAGg -5'
miRNA: 3'- gUGGGUCCCCCUGucGG---------GCCGAGagg -5'
miRNA: 3'- -GUgGGUCCCCCUGuCGgg---CCG-------AGAGG- -5'
miRNA: 3'- -GUGGGUCCCCCUGUCGGGCCGAGAGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9455 | 3' | -63.4 | NC_002531.1 | + | 81590 | 0.66 | 0.565171 |
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Target: 5'- aCACuCCAGGGGGG-AGCCCuuuGUuuaUUUCCu -3' miRNA: 3'- -GUG-GGUCCCCCUgUCGGGc--CG---AGAGG- -5' |
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| 9455 | 3' | -63.4 | NC_002531.1 | + | 37161 | 0.66 | 0.545909 |
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Target: 5'- aACUCAGGGGcacccACuGCCCGcCUCUCg -3' miRNA: 3'- gUGGGUCCCCc----UGuCGGGCcGAGAGg -5' |
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| 9455 | 3' | -63.4 | NC_002531.1 | + | 102987 | 0.68 | 0.444772 |
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Target: 5'- gGCCCAGGGGGAUcuCCauuuucuacUGGCUCa-- -3' miRNA: 3'- gUGGGUCCCCCUGucGG---------GCCGAGagg -5' |
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| 9455 | 3' | -63.4 | NC_002531.1 | + | 56054 | 0.7 | 0.311023 |
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Target: 5'- gCAgCCAGGGGGACuGCagacaGGCcaccucuUCUCCu -3' miRNA: 3'- -GUgGGUCCCCCUGuCGgg---CCG-------AGAGG- -5' |
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| 9455 | 3' | -63.4 | NC_002531.1 | + | 121923 | 1.11 | 0.000378 |
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Target: 5'- aCACCCAGGGGGACAGCCCGGCUCUCCg -3' miRNA: 3'- -GUGGGUCCCCCUGUCGGGCCGAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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