Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9456 | 5' | -53.2 | NC_002531.1 | + | 20238 | 0.66 | 0.965175 |
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Target: 5'- aUGGCCGAGGAUGA-UCCaaggGUgaUUUGGu -3' miRNA: 3'- -ACUGGUUCCUGCUcAGGga--CA--AGACC- -5' |
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| 9456 | 5' | -53.2 | NC_002531.1 | + | 90336 | 0.68 | 0.925683 |
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Target: 5'- --uCCAGGGGCGAGUgaCUGUcgUUGGa -3' miRNA: 3'- acuGGUUCCUGCUCAggGACAa-GACC- -5' |
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| 9456 | 5' | -53.2 | NC_002531.1 | + | 35315 | 0.68 | 0.901725 |
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Target: 5'- cGGCCAcaaacaGCGAGUCCCUGgacaUCUuGGg -3' miRNA: 3'- aCUGGUucc---UGCUCAGGGACa---AGA-CC- -5' |
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| 9456 | 5' | -53.2 | NC_002531.1 | + | 130039 | 0.69 | 0.865587 |
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Target: 5'- cGGCCGccccgucagggggGuuGGGgGGGUCCCUGU-CUGGg -3' miRNA: 3'- aCUGGU-------------U--CCUgCUCAGGGACAaGACC- -5' |
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| 9456 | 5' | -53.2 | NC_002531.1 | + | 7047 | 0.71 | 0.770545 |
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Target: 5'- aGAUUAGGGuGCGAGUUCCUGaugUUGGa -3' miRNA: 3'- aCUGGUUCC-UGCUCAGGGACaa-GACC- -5' |
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| 9456 | 5' | -53.2 | NC_002531.1 | + | 4356 | 0.74 | 0.627931 |
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Target: 5'- gUGAUCAAGGugGGGagagcUCCCUGU-CUGu -3' miRNA: 3'- -ACUGGUUCCugCUC-----AGGGACAaGACc -5' |
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| 9456 | 5' | -53.2 | NC_002531.1 | + | 122107 | 1.12 | 0.003075 |
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Target: 5'- aUGACCAAGGACGAGUCCCUGUUCUGGg -3' miRNA: 3'- -ACUGGUUCCUGCUCAGGGACAAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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