Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9458 | 5' | -47.3 | NC_002531.1 | + | 21824 | 0.66 | 0.999538 |
Target: 5'- uUGGGguGCU-GGGGUAGGCaUUGa-- -3' miRNA: 3'- -ACUCguCGAgCCUCAUUUGaAACauc -5' |
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9458 | 5' | -47.3 | NC_002531.1 | + | 13865 | 0.66 | 0.999101 |
Target: 5'- gGAGUAcccccacguGCUCGGGaccuccaguGUGGACUcUGUAGa -3' miRNA: 3'- aCUCGU---------CGAGCCU---------CAUUUGAaACAUC- -5' |
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9458 | 5' | -47.3 | NC_002531.1 | + | 105250 | 0.66 | 0.999101 |
Target: 5'- gGAGCAGCUgUGGcaccuACUUUGUAa -3' miRNA: 3'- aCUCGUCGA-GCCucauuUGAAACAUc -5' |
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9458 | 5' | -47.3 | NC_002531.1 | + | 87089 | 0.67 | 0.99835 |
Target: 5'- gUGcAGuCGGCUUgGGGGUGAACUUUuUAGg -3' miRNA: 3'- -AC-UC-GUCGAG-CCUCAUUUGAAAcAUC- -5' |
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9458 | 5' | -47.3 | NC_002531.1 | + | 54746 | 0.68 | 0.99441 |
Target: 5'- gUGGGUAGCUgGGgGGUAAGCUUa---- -3' miRNA: 3'- -ACUCGUCGAgCC-UCAUUUGAAacauc -5' |
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9458 | 5' | -47.3 | NC_002531.1 | + | 122951 | 1.1 | 0.014303 |
Target: 5'- cUGAGCAGCUCGGAGUAAACUUUGUAGu -3' miRNA: 3'- -ACUCGUCGAGCCUCAUUUGAAACAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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