Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9460 | 5' | -49.9 | NC_002531.1 | + | 71759 | 0.66 | 0.992521 |
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Target: 5'- gGGAGGUaCC-UgCUGcuAUGGGAAACUUa -3' miRNA: 3'- gUCUCCA-GGuAgGAC--UACCUUUUGAG- -5' |
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| 9460 | 5' | -49.9 | NC_002531.1 | + | 122589 | 0.66 | 0.991375 |
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Target: 5'- aCGGAGG-CCucguggCCUGcGUGGcGGAGCUCu -3' miRNA: 3'- -GUCUCCaGGua----GGAC-UACC-UUUUGAG- -5' |
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| 9460 | 5' | -49.9 | NC_002531.1 | + | 130167 | 0.67 | 0.988669 |
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Target: 5'- gGGAGGUCCGggggcaCCUGA-GGAaguuuaugcAAAUUCu -3' miRNA: 3'- gUCUCCAGGUa-----GGACUaCCU---------UUUGAG- -5' |
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| 9460 | 5' | -49.9 | NC_002531.1 | + | 61230 | 0.68 | 0.970894 |
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Target: 5'- uGGAGGagUCUGgggCCUGuuugGUGGAGGGCUCu -3' miRNA: 3'- gUCUCC--AGGUa--GGAC----UACCUUUUGAG- -5' |
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| 9460 | 5' | -49.9 | NC_002531.1 | + | 82656 | 0.68 | 0.970894 |
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Target: 5'- gUAGuGGaCCAggUCCUGAUcGGAGAGCaUCa -3' miRNA: 3'- -GUCuCCaGGU--AGGACUA-CCUUUUG-AG- -5' |
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| 9460 | 5' | -49.9 | NC_002531.1 | + | 92358 | 0.72 | 0.850633 |
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Target: 5'- uCAGAGGuuUCCucCCUGAUGGAAGAUc- -3' miRNA: 3'- -GUCUCC--AGGuaGGACUACCUUUUGag -5' |
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| 9460 | 5' | -49.9 | NC_002531.1 | + | 39442 | 0.73 | 0.832526 |
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Target: 5'- -uGAGGcCCAUCCUGG-GGAAGugauagcACUCa -3' miRNA: 3'- guCUCCaGGUAGGACUaCCUUU-------UGAG- -5' |
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| 9460 | 5' | -49.9 | NC_002531.1 | + | 124404 | 1.11 | 0.006504 |
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Target: 5'- gCAGAGGUCCAUCCUGAUGGAAAACUCa -3' miRNA: 3'- -GUCUCCAGGUAGGACUACCUUUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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