Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9461 | 3' | -55 | NC_002531.1 | + | 125027 | 0.66 | 0.912114 |
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Target: 5'- uCCUCUAUA-CuGUAGUCugUGUCCa- -3' miRNA: 3'- -GGAGAUGUaGcCAUCGGugACGGGag -5' |
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| 9461 | 3' | -55 | NC_002531.1 | + | 34199 | 0.66 | 0.899507 |
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Target: 5'- aCUC-AUGUaCGGacuacGGCCACUGCCCa- -3' miRNA: 3'- gGAGaUGUA-GCCa----UCGGUGACGGGag -5' |
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| 9461 | 3' | -55 | NC_002531.1 | + | 38433 | 0.66 | 0.899507 |
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Target: 5'- uCCgCUGC-UCGGgcaaucGGCguguGCUGCCCUCa -3' miRNA: 3'- -GGaGAUGuAGCCa-----UCGg---UGACGGGAG- -5' |
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| 9461 | 3' | -55 | NC_002531.1 | + | 113292 | 0.69 | 0.786678 |
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Target: 5'- aCCgUCUGCggCGaGUGGCUggugcgGCUGCCaCUCa -3' miRNA: 3'- -GG-AGAUGuaGC-CAUCGG------UGACGG-GAG- -5' |
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| 9461 | 3' | -55 | NC_002531.1 | + | 44578 | 0.7 | 0.718284 |
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Target: 5'- uUCUCUACAUauGUGuuucGCCACUGCCa-- -3' miRNA: 3'- -GGAGAUGUAgcCAU----CGGUGACGGgag -5' |
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| 9461 | 3' | -55 | NC_002531.1 | + | 29861 | 0.73 | 0.573367 |
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Target: 5'- --cCUGCAUCGGcauGCgCACUGCCCa- -3' miRNA: 3'- ggaGAUGUAGCCau-CG-GUGACGGGag -5' |
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| 9461 | 3' | -55 | NC_002531.1 | + | 124267 | 0.73 | 0.542683 |
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Target: 5'- aCCUCUGCAUcCGGaGGCCGuCUGUCUg- -3' miRNA: 3'- -GGAGAUGUA-GCCaUCGGU-GACGGGag -5' |
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| 9461 | 3' | -55 | NC_002531.1 | + | 124550 | 1.12 | 0.001761 |
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Target: 5'- cCCUCUACAUCGGUAGCCACUGCCCUCa -3' miRNA: 3'- -GGAGAUGUAGCCAUCGGUGACGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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