Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9461 | 5' | -49.6 | NC_002531.1 | + | 43503 | 0.66 | 0.992901 |
Target: 5'- gUGAGGGgGGCCGUGc-UUUUACc-- -3' miRNA: 3'- gACUCCCgUCGGUACauGAAAUGauu -5' |
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9461 | 5' | -49.6 | NC_002531.1 | + | 84860 | 0.67 | 0.979522 |
Target: 5'- uUGAGGGgAGagGUGUGCUgcACUGGa -3' miRNA: 3'- gACUCCCgUCggUACAUGAaaUGAUU- -5' |
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9461 | 5' | -49.6 | NC_002531.1 | + | 127309 | 0.68 | 0.964733 |
Target: 5'- -gGAGGGCAGgCGaGUACUUgUACUc- -3' miRNA: 3'- gaCUCCCGUCgGUaCAUGAA-AUGAuu -5' |
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9461 | 5' | -49.6 | NC_002531.1 | + | 6286 | 0.69 | 0.952918 |
Target: 5'- uUGAGcuaGGUAGCCucUGUGCUuUUGCUAAa -3' miRNA: 3'- gACUC---CCGUCGGu-ACAUGA-AAUGAUU- -5' |
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9461 | 5' | -49.6 | NC_002531.1 | + | 105633 | 0.69 | 0.938693 |
Target: 5'- aUGGGGuGCAG-CGUGUGCUcugcuuUUGCUGAg -3' miRNA: 3'- gACUCC-CGUCgGUACAUGA------AAUGAUU- -5' |
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9461 | 5' | -49.6 | NC_002531.1 | + | 124515 | 1.07 | 0.010253 |
Target: 5'- gCUGAGGGCAGCCAUGUACUUUACUAAg -3' miRNA: 3'- -GACUCCCGUCGGUACAUGAAAUGAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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