Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9463 | 3' | -55.6 | NC_002531.1 | + | 63601 | 0.67 | 0.858709 |
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Target: 5'- cGugCCugAGGG-CCUCaggaaUGGGGGGa -3' miRNA: 3'- -CugGGugUCCUaGGAGaag--AUCCCCU- -5' |
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| 9463 | 3' | -55.6 | NC_002531.1 | + | 29261 | 0.67 | 0.842639 |
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Target: 5'- cGCCCACuagAGuGAUCCUgaUCUuaguauugaagGGGGGAg -3' miRNA: 3'- cUGGGUG---UC-CUAGGAgaAGA-----------UCCCCU- -5' |
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| 9463 | 3' | -55.6 | NC_002531.1 | + | 64942 | 0.67 | 0.833459 |
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Target: 5'- -uCCCAgcaGGGAUUCUCUUUUuuugcucGGGGGGg -3' miRNA: 3'- cuGGGUg--UCCUAGGAGAAGA-------UCCCCU- -5' |
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| 9463 | 3' | -55.6 | NC_002531.1 | + | 49293 | 0.68 | 0.808186 |
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Target: 5'- aACCC-CuGGG-CCUCUcaaaaaggcucUCUGGGGGAu -3' miRNA: 3'- cUGGGuGuCCUaGGAGA-----------AGAUCCCCU- -5' |
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| 9463 | 3' | -55.6 | NC_002531.1 | + | 391 | 0.69 | 0.741895 |
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Target: 5'- gGGCCCgaGCAGGGagCUaaugaUCUGGGGGAc -3' miRNA: 3'- -CUGGG--UGUCCUagGAga---AGAUCCCCU- -5' |
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| 9463 | 3' | -55.6 | NC_002531.1 | + | 130074 | 1.09 | 0.002594 |
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Target: 5'- cGACCCACAGGAUCCUCUUCUAGGGGAc -3' miRNA: 3'- -CUGGGUGUCCUAGGAGAAGAUCCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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