Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9463 | 5' | -51.7 | NC_002531.1 | + | 35128 | 0.66 | 0.977865 |
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Target: 5'- -aUCACCgcugugcaGAAGAGGC-CCUGGGc-- -3' miRNA: 3'- ugAGUGGa-------CUUUUCCGuGGACCUuca -5' |
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| 9463 | 5' | -51.7 | NC_002531.1 | + | 78669 | 0.66 | 0.975287 |
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Target: 5'- uGCUCuCCUGcugugagcaAGAGGGUAuuaguuaauCCUGGggGUg -3' miRNA: 3'- -UGAGuGGAC---------UUUUCCGU---------GGACCuuCA- -5' |
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| 9463 | 5' | -51.7 | NC_002531.1 | + | 112144 | 0.67 | 0.962412 |
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Target: 5'- uGCcCACCUGuuucagauaauguGGAGGGCGCCUGuGGAa- -3' miRNA: 3'- -UGaGUGGAC-------------UUUUCCGUGGAC-CUUca -5' |
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| 9463 | 5' | -51.7 | NC_002531.1 | + | 112179 | 0.67 | 0.955094 |
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Target: 5'- uACUCAUCUGAAacuagGAGGCAUCaguuacagacaUGaGGAGUg -3' miRNA: 3'- -UGAGUGGACUU-----UUCCGUGG-----------AC-CUUCA- -5' |
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| 9463 | 5' | -51.7 | NC_002531.1 | + | 15425 | 0.69 | 0.914054 |
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Target: 5'- uCUCGCCuUGGuuGGGCACCacuGAAGUu -3' miRNA: 3'- uGAGUGG-ACUuuUCCGUGGac-CUUCA- -5' |
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| 9463 | 5' | -51.7 | NC_002531.1 | + | 58241 | 0.72 | 0.744565 |
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Target: 5'- gGCU-ACCaGGAAAGGUACCaUGGAGGUc -3' miRNA: 3'- -UGAgUGGaCUUUUCCGUGG-ACCUUCA- -5' |
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| 9463 | 5' | -51.7 | NC_002531.1 | + | 123371 | 0.73 | 0.723906 |
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Target: 5'- cGCUCgcGCCUGgcAGGGUAuaguCCUGGggGUu -3' miRNA: 3'- -UGAG--UGGACuuUUCCGU----GGACCuuCA- -5' |
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| 9463 | 5' | -51.7 | NC_002531.1 | + | 130040 | 1.08 | 0.006158 |
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Target: 5'- aACUCACCUGAAAAGGCACCUGGAAGUa -3' miRNA: 3'- -UGAGUGGACUUUUCCGUGGACCUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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