Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9464 | 3' | -58.4 | NC_002531.1 | + | 91109 | 0.66 | 0.733891 |
Target: 5'- cCUCUCUGCCaagucugGUGUG-GGCUguaauguuaCCUCAUu -3' miRNA: 3'- cGAGAGACGG-------CACGCuCCGA---------GGAGUG- -5' |
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9464 | 3' | -58.4 | NC_002531.1 | + | 77542 | 0.67 | 0.69507 |
Target: 5'- cGCUCgg-GCCGUGaUGAGGgUCCUa-- -3' miRNA: 3'- -CGAGagaCGGCAC-GCUCCgAGGAgug -5' |
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9464 | 3' | -58.4 | NC_002531.1 | + | 42913 | 0.67 | 0.674817 |
Target: 5'- aUUCUaugCUGCCgGUGCGGGGUUUC-CACc -3' miRNA: 3'- cGAGA---GACGG-CACGCUCCGAGGaGUG- -5' |
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9464 | 3' | -58.4 | NC_002531.1 | + | 23828 | 0.69 | 0.572775 |
Target: 5'- uGUgagUUCUGCCGaG-GAGGCUCCUCu- -3' miRNA: 3'- -CGa--GAGACGGCaCgCUCCGAGGAGug -5' |
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9464 | 3' | -58.4 | NC_002531.1 | + | 89988 | 0.7 | 0.503363 |
Target: 5'- aGCcCUCaUGCCcacUGUG-GGCUCCUCGCu -3' miRNA: 3'- -CGaGAG-ACGGc--ACGCuCCGAGGAGUG- -5' |
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9464 | 3' | -58.4 | NC_002531.1 | + | 81 | 1.11 | 0.000903 |
Target: 5'- gGCUCUCUGCCGUGCGAGGCUCCUCACa -3' miRNA: 3'- -CGAGAGACGGCACGCUCCGAGGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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