miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9467 3' -59.5 NC_002531.1 + 103563 0.66 0.70778
Target:  5'- -gGGAgUGGUGGCggCGCC-GGAGGCGCc -3'
miRNA:   3'- ugUCU-GUCACUG--GCGGaCCUCCGCGa -5'
9467 3' -59.5 NC_002531.1 + 90985 0.66 0.70778
Target:  5'- cCAGugAgcGUGGCgGCCuauaUGGAGGUGUg -3'
miRNA:   3'- uGUCugU--CACUGgCGG----ACCUCCGCGa -5'
9467 3' -59.5 NC_002531.1 + 45748 0.68 0.576105
Target:  5'- ---aACAGUGugCGCUUuGAGGUGCUa -3'
miRNA:   3'- ugucUGUCACugGCGGAcCUCCGCGA- -5'
9467 3' -59.5 NC_002531.1 + 103957 0.69 0.535195
Target:  5'- gGCAG-CGGUGgaaguggGCCcCCUGGAGGCaGCg -3'
miRNA:   3'- -UGUCuGUCAC-------UGGcGGACCUCCG-CGa -5'
9467 3' -59.5 NC_002531.1 + 99194 0.69 0.506883
Target:  5'- aGCGGACAGgGACUGUgaGGGGG-GCUc -3'
miRNA:   3'- -UGUCUGUCaCUGGCGgaCCUCCgCGA- -5'
9467 3' -59.5 NC_002531.1 + 123367 0.71 0.423639
Target:  5'- cAUAGACAgauGUGGCaaugGCCUGGuGGUGCa -3'
miRNA:   3'- -UGUCUGU---CACUGg---CGGACCuCCGCGa -5'
9467 3' -59.5 NC_002531.1 + 12186 1.06 0.001595
Target:  5'- aACAGACAGUGACCGCCUGGAGGCGCUc -3'
miRNA:   3'- -UGUCUGUCACUGGCGGACCUCCGCGA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.