Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9468 | 5' | -55.2 | NC_002531.1 | + | 35135 | 0.66 | 0.883582 |
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Target: 5'- cUguGcAGAAga-GGCCCuGGGCUACAGa -3' miRNA: 3'- -AguC-UCUUaggUCGGGuCCCGAUGUU- -5' |
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| 9468 | 5' | -55.2 | NC_002531.1 | + | 49453 | 0.66 | 0.852789 |
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Target: 5'- cCAGAGAG-CCuuuuugagaGGCCCAGGGgUucACAGu -3' miRNA: 3'- aGUCUCUUaGG---------UCGGGUCCCgA--UGUU- -5' |
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| 9468 | 5' | -55.2 | NC_002531.1 | + | 37631 | 0.67 | 0.833495 |
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Target: 5'- cUAGAGGAUCUAGCUaacauuuuaguggaCAGGGCccaACAAc -3' miRNA: 3'- aGUCUCUUAGGUCGG--------------GUCCCGa--UGUU- -5' |
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| 9468 | 5' | -55.2 | NC_002531.1 | + | 69866 | 0.68 | 0.76587 |
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Target: 5'- gCAGAGGAaggagauuccgauccUCCgauaaAGCCCguaAGGGCUGCGc -3' miRNA: 3'- aGUCUCUU---------------AGG-----UCGGG---UCCCGAUGUu -5' |
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| 9468 | 5' | -55.2 | NC_002531.1 | + | 8539 | 0.69 | 0.741957 |
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Target: 5'- aUCcGuGAuGUCCAGCCCAGGGgUAgAGu -3' miRNA: 3'- -AGuCuCU-UAGGUCGGGUCCCgAUgUU- -5' |
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| 9468 | 5' | -55.2 | NC_002531.1 | + | 13181 | 1.07 | 0.003048 |
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Target: 5'- gUCAGAGAAUCCAGCCCAGGGCUACAAg -3' miRNA: 3'- -AGUCUCUUAGGUCGGGUCCCGAUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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