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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9470 | 5' | -46.9 | NC_002531.1 | + | 125770 | 0.67 | 0.998762 |
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Target: 5'- gGUGUCACagcaaaggaaagCUgcgggUGGUCUAAGGCAg- -3' miRNA: 3'- -UACAGUGg-----------GAaa---ACCAGAUUUCGUac -5' |
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| 9470 | 5' | -46.9 | NC_002531.1 | + | 17601 | 0.67 | 0.997856 |
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Target: 5'- gGUGUCACCUaugUUGGUgaUAAAGUAg- -3' miRNA: 3'- -UACAGUGGGaa-AACCAg-AUUUCGUac -5' |
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| 9470 | 5' | -46.9 | NC_002531.1 | + | 58301 | 0.67 | 0.997856 |
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Target: 5'- aAUGUCaACCUUUUuggGGUC-AAAGCGUa -3' miRNA: 3'- -UACAG-UGGGAAAa--CCAGaUUUCGUAc -5' |
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| 9470 | 5' | -46.9 | NC_002531.1 | + | 50267 | 0.68 | 0.99565 |
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Target: 5'- -aGUUGCUCUcUUGGUCaaaaagugGAGGCAUGa -3' miRNA: 3'- uaCAGUGGGAaAACCAGa-------UUUCGUAC- -5' |
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| 9470 | 5' | -46.9 | NC_002531.1 | + | 124517 | 0.69 | 0.990595 |
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Target: 5'- -cGUCACCUggaugauGUCUGAGGCAUa -3' miRNA: 3'- uaCAGUGGGaaaac--CAGAUUUCGUAc -5' |
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| 9470 | 5' | -46.9 | NC_002531.1 | + | 17477 | 1.09 | 0.016158 |
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Target: 5'- gAUGUCACCCUUUUGGUCUAAAGCAUGg -3' miRNA: 3'- -UACAGUGGGAAAACCAGAUUUCGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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