Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9471 | 3' | -51.7 | NC_002531.1 | + | 120082 | 0.66 | 0.974433 |
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Target: 5'- --aGUAacgGGAGAaGGCccAGGCGGAGGa -3' miRNA: 3'- uaaCAUg--CCUCUaCCGc-UUCGUCUCC- -5' |
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| 9471 | 3' | -51.7 | NC_002531.1 | + | 119791 | 0.67 | 0.953997 |
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Target: 5'- --cGUAacaGGAGAaGGCGgAAGCggaccAGAGGg -3' miRNA: 3'- uaaCAUg--CCUCUaCCGC-UUCG-----UCUCC- -5' |
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| 9471 | 3' | -51.7 | NC_002531.1 | + | 69887 | 0.68 | 0.945249 |
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Target: 5'- --aGUGaugUGGaAGAUGGgGguGCAGAGGa -3' miRNA: 3'- uaaCAU---GCC-UCUACCgCuuCGUCUCC- -5' |
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| 9471 | 3' | -51.7 | NC_002531.1 | + | 103558 | 0.68 | 0.9302 |
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Target: 5'- cUUGUG-GGAGugGUGGCGgcGcCGGAGGc -3' miRNA: 3'- uAACAUgCCUC--UACCGCuuC-GUCUCC- -5' |
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| 9471 | 3' | -51.7 | NC_002531.1 | + | 74347 | 0.7 | 0.893076 |
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Target: 5'- -aUGUAUGGGGGUGGUc-AGCAGAc- -3' miRNA: 3'- uaACAUGCCUCUACCGcuUCGUCUcc -5' |
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| 9471 | 3' | -51.7 | NC_002531.1 | + | 90326 | 0.72 | 0.783782 |
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Target: 5'- --gGUAUGGAGAgggGGCagcgGGAGCGGAGc -3' miRNA: 3'- uaaCAUGCCUCUa--CCG----CUUCGUCUCc -5' |
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| 9471 | 3' | -51.7 | NC_002531.1 | + | 37377 | 0.72 | 0.774073 |
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Target: 5'- --gGUGCugaGGGGAUGGCuGggGCAGguuGGGg -3' miRNA: 3'- uaaCAUG---CCUCUACCG-CuuCGUC---UCC- -5' |
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| 9471 | 3' | -51.7 | NC_002531.1 | + | 26328 | 1.1 | 0.005397 |
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Target: 5'- cAUUGUACGGAGAUGGCGAAGCAGAGGa -3' miRNA: 3'- -UAACAUGCCUCUACCGCUUCGUCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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