Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9474 | 3' | -47.5 | NC_002531.1 | + | 24144 | 0.66 | 0.999599 |
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Target: 5'- aCCcACCAGAG-ACgaugguugGGCAGAGG-CUg -3' miRNA: 3'- -GGaUGGUCUCaUGaa------CCGUUUCUaGA- -5' |
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| 9474 | 3' | -47.5 | NC_002531.1 | + | 21365 | 0.67 | 0.998812 |
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Target: 5'- -aUGCUGGGGUACaagcUGGCAAAG-UCa -3' miRNA: 3'- ggAUGGUCUCAUGa---ACCGUUUCuAGa -5' |
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| 9474 | 3' | -47.5 | NC_002531.1 | + | 98346 | 0.67 | 0.998551 |
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Target: 5'- gCUGCCcuGGAGaGCUUGGCA---GUCa -3' miRNA: 3'- gGAUGG--UCUCaUGAACCGUuucUAGa -5' |
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| 9474 | 3' | -47.5 | NC_002531.1 | + | 122496 | 0.68 | 0.995689 |
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Target: 5'- aCCUGuuugcugagagccCCGGGGUGg-UGGUGGAGAUCUc -3' miRNA: 3'- -GGAU-------------GGUCUCAUgaACCGUUUCUAGA- -5' |
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| 9474 | 3' | -47.5 | NC_002531.1 | + | 94545 | 0.69 | 0.989594 |
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Target: 5'- gCUUGCgCAGuGUGCUuaaaaaUGGCGGAGcAUCUg -3' miRNA: 3'- -GGAUG-GUCuCAUGA------ACCGUUUC-UAGA- -5' |
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| 9474 | 3' | -47.5 | NC_002531.1 | + | 27267 | 1.1 | 0.013686 |
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Target: 5'- uCCUACCAGAGUACUUGGCAAAGAUCUu -3' miRNA: 3'- -GGAUGGUCUCAUGAACCGUUUCUAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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