Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9474 | 5' | -52.3 | NC_002531.1 | + | 7984 | 0.66 | 0.968279 |
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Target: 5'- aGCuGAGUCUacuuuuaGCCAAGAGUUUUUAa -3' miRNA: 3'- -UGuCUCGGAag-----CGGUUCUCGAGAAUc -5' |
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| 9474 | 5' | -52.3 | NC_002531.1 | + | 12001 | 0.66 | 0.968279 |
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Target: 5'- gUAGAGCCUUCGCaggcuGGAuuUUUUAGu -3' miRNA: 3'- uGUCUCGGAAGCGgu---UCUcgAGAAUC- -5' |
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| 9474 | 5' | -52.3 | NC_002531.1 | + | 80071 | 0.66 | 0.957611 |
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Target: 5'- gACAGAggagGCCaUUGCUAcucaguuuuuAGAGCUCUUAc -3' miRNA: 3'- -UGUCU----CGGaAGCGGU----------UCUCGAGAAUc -5' |
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| 9474 | 5' | -52.3 | NC_002531.1 | + | 76591 | 0.69 | 0.877391 |
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Target: 5'- gAUAGcuGCCUUUGCCcuGGGCUgCUUGGc -3' miRNA: 3'- -UGUCu-CGGAAGCGGuuCUCGA-GAAUC- -5' |
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| 9474 | 5' | -52.3 | NC_002531.1 | + | 37626 | 0.7 | 0.819166 |
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Target: 5'- uACAGGGCUgcgcugCGCCGGGA-CUCUUGa -3' miRNA: 3'- -UGUCUCGGaa----GCGGUUCUcGAGAAUc -5' |
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| 9474 | 5' | -52.3 | NC_002531.1 | + | 122964 | 0.77 | 0.468794 |
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Target: 5'- uGCcGGGCCUUCGCUGAGcAGCUCggAGu -3' miRNA: 3'- -UGuCUCGGAAGCGGUUC-UCGAGaaUC- -5' |
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| 9474 | 5' | -52.3 | NC_002531.1 | + | 27302 | 1.08 | 0.005362 |
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Target: 5'- cACAGAGCCUUCGCCAAGAGCUCUUAGc -3' miRNA: 3'- -UGUCUCGGAAGCGGUUCUCGAGAAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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