Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9475 | 5' | -44.8 | NC_002531.1 | + | 74612 | 0.66 | 0.999981 |
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Target: 5'- -gAGCGGUg-CUGCUGcGGCUGGuCGCa -3' miRNA: 3'- aaUUGUUAagGACGACaUUGACU-GUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 24056 | 0.66 | 0.999974 |
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Target: 5'- -cAGCGcgUCCUGCaGc--CUGGCGCu -3' miRNA: 3'- aaUUGUuaAGGACGaCauuGACUGUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 28075 | 0.66 | 0.999964 |
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Target: 5'- -aAAC-AUUCCggGCUuUAACUGACAa -3' miRNA: 3'- aaUUGuUAAGGa-CGAcAUUGACUGUg -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 81858 | 0.66 | 0.999951 |
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Target: 5'- -gAugGAguggaCCUGCUGcgGGCUGugGCu -3' miRNA: 3'- aaUugUUaa---GGACGACa-UUGACugUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 7401 | 0.66 | 0.999941 |
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Target: 5'- cUGACAGUUCUUGCUGgccccauccACACa -3' miRNA: 3'- aAUUGUUAAGGACGACauugac---UGUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 60380 | 0.68 | 0.999299 |
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Target: 5'- -gGugAAUUCCacggUGUUGUGGuCUGACAUa -3' miRNA: 3'- aaUugUUAAGG----ACGACAUU-GACUGUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 68058 | 0.7 | 0.997577 |
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Target: 5'- --------cCCUGgaGUGGCUGGCGCg -3' miRNA: 3'- aauuguuaaGGACgaCAUUGACUGUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 24782 | 0.71 | 0.992099 |
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Target: 5'- gUGGCAGUuggCCUGCUGUuauACUgcaacGACGCu -3' miRNA: 3'- aAUUGUUAa--GGACGACAu--UGA-----CUGUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 109882 | 0.77 | 0.90572 |
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Target: 5'- ----uGGUUCUUGCUGUGACUGAaaCACu -3' miRNA: 3'- aauugUUAAGGACGACAUUGACU--GUG- -5' |
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| 9475 | 5' | -44.8 | NC_002531.1 | + | 27953 | 1.1 | 0.021649 |
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Target: 5'- aUUAACAAUUCCUGCUGUAACUGACACa -3' miRNA: 3'- -AAUUGUUAAGGACGACAUUGACUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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