Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9478 | 5' | -43.7 | NC_002531.1 | + | 122512 | 0.66 | 0.999991 |
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Target: 5'- -------gAUAGCGaacaGGGCGGCCAUUu -3' miRNA: 3'- uguuuuuaUAUCGUg---UCCGUUGGUAA- -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 84370 | 0.67 | 0.999974 |
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Target: 5'- uCAAAAAcAUGGCucaGCAGGCAAUUGUg -3' miRNA: 3'- uGUUUUUaUAUCG---UGUCCGUUGGUAa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 37905 | 0.67 | 0.999964 |
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Target: 5'- -gGAAGAUGUGGCcugUAGGCAGCUc-- -3' miRNA: 3'- ugUUUUUAUAUCGu--GUCCGUUGGuaa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 90445 | 0.67 | 0.99995 |
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Target: 5'- cACAGAGAU-UGGCAgAGGCGAUgGc- -3' miRNA: 3'- -UGUUUUUAuAUCGUgUCCGUUGgUaa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 12039 | 0.67 | 0.999949 |
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Target: 5'- uACAGu-GUGUAGUACAGGUacucgcuGACCGc- -3' miRNA: 3'- -UGUUuuUAUAUCGUGUCCG-------UUGGUaa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 37785 | 0.68 | 0.999909 |
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Target: 5'- uCAAGAGUcccGGCGCAGcGCAGCCc-- -3' miRNA: 3'- uGUUUUUAua-UCGUGUC-CGUUGGuaa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 29980 | 0.68 | 0.999838 |
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Target: 5'- gGCGAAGAUuuuGCAagucccuacCAGGUAGCCGUg -3' miRNA: 3'- -UGUUUUUAuauCGU---------GUCCGUUGGUAa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 59839 | 0.73 | 0.990343 |
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Target: 5'- ---uAGAUGUAGUGCAGGCGugCAc- -3' miRNA: 3'- uguuUUUAUAUCGUGUCCGUugGUaa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 31871 | 0.76 | 0.958226 |
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Target: 5'- aACAGAAGg--GGCauaACAGGCAGCCAc- -3' miRNA: 3'- -UGUUUUUauaUCG---UGUCCGUUGGUaa -5' |
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| 9478 | 5' | -43.7 | NC_002531.1 | + | 28842 | 1.07 | 0.04095 |
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Target: 5'- aACAAAAAUAUAGCACAGGCAACCAUUa -3' miRNA: 3'- -UGUUUUUAUAUCGUGUCCGUUGGUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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