Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9479 | 3' | -54.9 | NC_002531.1 | + | 86377 | 0.66 | 0.90434 |
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Target: 5'- cUGCCAGgcugaaaaggcaaUAUACUUG-GCCCGUuuGGUGUa -3' miRNA: 3'- -ACGGUC-------------AUGUGAGCgUGGGCA--UCACA- -5' |
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| 9479 | 3' | -54.9 | NC_002531.1 | + | 29341 | 0.66 | 0.898484 |
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Target: 5'- -uCCAGUACACaaGCuuccCCCGUAGUu- -3' miRNA: 3'- acGGUCAUGUGagCGu---GGGCAUCAca -5' |
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| 9479 | 3' | -54.9 | NC_002531.1 | + | 24572 | 0.66 | 0.898484 |
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Target: 5'- aGCCGaaggACACUCGUGCCCG-GGUc- -3' miRNA: 3'- aCGGUca--UGUGAGCGUGGGCaUCAca -5' |
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| 9479 | 3' | -54.9 | NC_002531.1 | + | 49029 | 0.67 | 0.88477 |
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Target: 5'- aGCCGGgguCACUaCGCugCUGUAGc-- -3' miRNA: 3'- aCGGUCau-GUGA-GCGugGGCAUCaca -5' |
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| 9479 | 3' | -54.9 | NC_002531.1 | + | 24753 | 0.71 | 0.653506 |
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Target: 5'- aGCCAGUGaaGCUgCGCACCUGUgaaccAGUGg -3' miRNA: 3'- aCGGUCAUg-UGA-GCGUGGGCA-----UCACa -5' |
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| 9479 | 3' | -54.9 | NC_002531.1 | + | 35643 | 0.72 | 0.622073 |
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Target: 5'- gGCCGG-GCGCUCGCAgCUGUGGcUGc -3' miRNA: 3'- aCGGUCaUGUGAGCGUgGGCAUC-ACa -5' |
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| 9479 | 3' | -54.9 | NC_002531.1 | + | 9724 | 0.72 | 0.580311 |
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Target: 5'- aGCCAGgUGCAgcaucUUCGCGCCUGUuGUGUc -3' miRNA: 3'- aCGGUC-AUGU-----GAGCGUGGGCAuCACA- -5' |
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| 9479 | 3' | -54.9 | NC_002531.1 | + | 29149 | 1.07 | 0.003752 |
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Target: 5'- aUGCCAGUACACUCGCACCCGUAGUGUu -3' miRNA: 3'- -ACGGUCAUGUGAGCGUGGGCAUCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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