Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9481 | 3' | -55.7 | NC_002531.1 | + | 104003 | 0.67 | 0.73613 |
Target: 5'- -cGGGGCAGGCC-CUcaaaaUGCACcUGguUg -3' miRNA: 3'- aaCUCCGUCCGGuGA-----ACGUGaACguA- -5' |
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9481 | 3' | -55.7 | NC_002531.1 | + | 54719 | 0.68 | 0.725776 |
Target: 5'- -aGAGGCGucacuccuaauGGCCACUccuaGUACUUGCu- -3' miRNA: 3'- aaCUCCGU-----------CCGGUGAa---CGUGAACGua -5' |
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9481 | 3' | -55.7 | NC_002531.1 | + | 36724 | 0.68 | 0.715333 |
Target: 5'- -cGAcGGCAGGCgAC-UGCACUUGa-- -3' miRNA: 3'- aaCU-CCGUCCGgUGaACGUGAACgua -5' |
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9481 | 3' | -55.7 | NC_002531.1 | + | 29840 | 1.03 | 0.004127 |
Target: 5'- gUUGAGGCAGGCCACUUGCACUUGCAUu -3' miRNA: 3'- -AACUCCGUCCGGUGAACGUGAACGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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