Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9481 | 5' | -52.2 | NC_002531.1 | + | 62102 | 0.66 | 0.948227 |
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Target: 5'- aAAGUGUGGGUGuGAGAUgcugAGCUUGgUUu -3' miRNA: 3'- -UUUACGUCCACuCUCUA----UCGGACgAA- -5' |
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| 9481 | 5' | -52.2 | NC_002531.1 | + | 37501 | 0.66 | 0.93321 |
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Target: 5'- ---gGguGGUGAGAuagucgguGAUAGCCUGg-- -3' miRNA: 3'- uuuaCguCCACUCU--------CUAUCGGACgaa -5' |
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| 9481 | 5' | -52.2 | NC_002531.1 | + | 115862 | 0.66 | 0.93321 |
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Target: 5'- -uAUGUAGGUGGGGGcUGGCCccguccccggGCUg -3' miRNA: 3'- uuUACGUCCACUCUCuAUCGGa---------CGAa -5' |
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| 9481 | 5' | -52.2 | NC_002531.1 | + | 67308 | 0.67 | 0.902654 |
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Target: 5'- gGAGUGCAGGgucuuUGAGAGAUucuGUgCUGCa- -3' miRNA: 3'- -UUUACGUCC-----ACUCUCUAu--CG-GACGaa -5' |
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| 9481 | 5' | -52.2 | NC_002531.1 | + | 23430 | 0.69 | 0.822017 |
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Target: 5'- --uUGUGGGUGAu-GAUAGCCUGUg- -3' miRNA: 3'- uuuACGUCCACUcuCUAUCGGACGaa -5' |
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| 9481 | 5' | -52.2 | NC_002531.1 | + | 29874 | 1.03 | 0.009322 |
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Target: 5'- cAAAUGCAGGUGAGAGAUAGCCUGCUUu -3' miRNA: 3'- -UUUACGUCCACUCUCUAUCGGACGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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