Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9485 | 5' | -58 | NC_002531.1 | + | 104917 | 0.66 | 0.768834 |
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Target: 5'- gGCc-CUGAGguGCGCUCUCaGCCaCCAa -3' miRNA: 3'- -CGacGACUCguUGUGAGGGaCGG-GGU- -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 32461 | 0.66 | 0.759257 |
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Target: 5'- aGCUGCUgcGAGCGcCACUCaCCa-CCCUAg -3' miRNA: 3'- -CGACGA--CUCGUuGUGAG-GGacGGGGU- -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 51444 | 0.67 | 0.699713 |
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Target: 5'- --aGuCUGAGCAACGCUUCUUauaccCCCCAg -3' miRNA: 3'- cgaC-GACUCGUUGUGAGGGAc----GGGGU- -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 24793 | 0.67 | 0.689531 |
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Target: 5'- cCUGCUGuuauacuGCAacgACGCUCaCCUGCCUUc -3' miRNA: 3'- cGACGACu------CGU---UGUGAG-GGACGGGGu -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 38431 | 0.68 | 0.65873 |
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Target: 5'- cGCUGCUcGGGCAAUcgGCgugUgCUGCCCuCAa -3' miRNA: 3'- -CGACGA-CUCGUUG--UGa--GgGACGGG-GU- -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 73920 | 0.68 | 0.638072 |
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Target: 5'- -gUGCUGcAGCAAgACUCUCaGCCCa- -3' miRNA: 3'- cgACGAC-UCGUUgUGAGGGaCGGGgu -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 24217 | 0.71 | 0.4461 |
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Target: 5'- aGCUGUaGAGCGccaggcugcaggacGCGCUgCCUGCUCCu -3' miRNA: 3'- -CGACGaCUCGU--------------UGUGAgGGACGGGGu -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 54306 | 0.74 | 0.308883 |
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Target: 5'- --aGCUGGGCgAGCGCUCCaagGCCCUAu -3' miRNA: 3'- cgaCGACUCG-UUGUGAGGga-CGGGGU- -5' |
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| 9485 | 5' | -58 | NC_002531.1 | + | 32336 | 1.1 | 0.001037 |
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Target: 5'- aGCUGCUGAGCAACACUCCCUGCCCCAg -3' miRNA: 3'- -CGACGACUCGUUGUGAGGGACGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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