Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9487 | 5' | -56.3 | NC_002531.1 | + | 42552 | 0.66 | 0.83552 |
Target: 5'- --cAGCCagGGGUgGCAGGcgCUCUGCa -3' miRNA: 3'- gacUCGGggUCCAaCGUCCa-GAGAUG- -5' |
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9487 | 5' | -56.3 | NC_002531.1 | + | 36972 | 0.67 | 0.809384 |
Target: 5'- uUGGGgCCCAGGUUGUcuAGGaguucuUCUCgagagGCg -3' miRNA: 3'- gACUCgGGGUCCAACG--UCC------AGAGa----UG- -5' |
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9487 | 5' | -56.3 | NC_002531.1 | + | 80528 | 0.67 | 0.803968 |
Target: 5'- aUGAGCCCgGGGUgaaaggugacauuccUGUAGGUgUUgGCu -3' miRNA: 3'- gACUCGGGgUCCA---------------ACGUCCAgAGaUG- -5' |
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9487 | 5' | -56.3 | NC_002531.1 | + | 122484 | 0.7 | 0.640329 |
Target: 5'- -aGAGCCCCGGGguggUGguGGagaUCUCc-- -3' miRNA: 3'- gaCUCGGGGUCCa---ACguCC---AGAGaug -5' |
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9487 | 5' | -56.3 | NC_002531.1 | + | 129469 | 0.7 | 0.629878 |
Target: 5'- -cGAGCCCCuAGGUccuuggGCGgGGUCUugcCUGCa -3' miRNA: 3'- gaCUCGGGG-UCCAa-----CGU-CCAGA---GAUG- -5' |
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9487 | 5' | -56.3 | NC_002531.1 | + | 24211 | 0.7 | 0.608989 |
Target: 5'- -aGAGCgCCAGGcUGCAGGaCgcgCUGCc -3' miRNA: 3'- gaCUCGgGGUCCaACGUCCaGa--GAUG- -5' |
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9487 | 5' | -56.3 | NC_002531.1 | + | 50616 | 1.08 | 0.002146 |
Target: 5'- uCUGAGCCCCAGGUUGCAGGUCUCUACc -3' miRNA: 3'- -GACUCGGGGUCCAACGUCCAGAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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