miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
9487 5' -56.3 NC_002531.1 + 42552 0.66 0.83552
Target:  5'- --cAGCCagGGGUgGCAGGcgCUCUGCa -3'
miRNA:   3'- gacUCGGggUCCAaCGUCCa-GAGAUG- -5'
9487 5' -56.3 NC_002531.1 + 36972 0.67 0.809384
Target:  5'- uUGGGgCCCAGGUUGUcuAGGaguucuUCUCgagagGCg -3'
miRNA:   3'- gACUCgGGGUCCAACG--UCC------AGAGa----UG- -5'
9487 5' -56.3 NC_002531.1 + 80528 0.67 0.803968
Target:  5'- aUGAGCCCgGGGUgaaaggugacauuccUGUAGGUgUUgGCu -3'
miRNA:   3'- gACUCGGGgUCCA---------------ACGUCCAgAGaUG- -5'
9487 5' -56.3 NC_002531.1 + 122484 0.7 0.640329
Target:  5'- -aGAGCCCCGGGguggUGguGGagaUCUCc-- -3'
miRNA:   3'- gaCUCGGGGUCCa---ACguCC---AGAGaug -5'
9487 5' -56.3 NC_002531.1 + 129469 0.7 0.629878
Target:  5'- -cGAGCCCCuAGGUccuuggGCGgGGUCUugcCUGCa -3'
miRNA:   3'- gaCUCGGGG-UCCAa-----CGU-CCAGA---GAUG- -5'
9487 5' -56.3 NC_002531.1 + 24211 0.7 0.608989
Target:  5'- -aGAGCgCCAGGcUGCAGGaCgcgCUGCc -3'
miRNA:   3'- gaCUCGgGGUCCaACGUCCaGa--GAUG- -5'
9487 5' -56.3 NC_002531.1 + 50616 1.08 0.002146
Target:  5'- uCUGAGCCCCAGGUUGCAGGUCUCUACc -3'
miRNA:   3'- -GACUCGGGGUCCAACGUCCAGAGAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.