Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 9488 | 3' | -56.2 | NC_002531.1 | + | 18320 | 0.7 | 0.662282 |
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Target: 5'- cUGCucaCGGCACCAC-GCGCGGgauagCUCUu -3' miRNA: 3'- -AUGug-GUCGUGGUGuCGUGCCa----GAGG- -5' |
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| 9488 | 3' | -56.2 | NC_002531.1 | + | 14577 | 0.69 | 0.723726 |
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Target: 5'- -cCACCAGCucaCGCAGCugGucuagcuUCUCCa -3' miRNA: 3'- auGUGGUCGug-GUGUCGugCc------AGAGG- -5' |
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| 9488 | 3' | -56.2 | NC_002531.1 | + | 14027 | 0.68 | 0.772775 |
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Target: 5'- cACACUggaggucccgAGCAcguggggguacuCCAgGGCACaGGUCUCCu -3' miRNA: 3'- aUGUGG----------UCGU------------GGUgUCGUG-CCAGAGG- -5' |
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| 9488 | 3' | -56.2 | NC_002531.1 | + | 12075 | 0.68 | 0.753477 |
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Target: 5'- cGCugUGGCgGCCG-AGCugGGUCUCa -3' miRNA: 3'- aUGugGUCG-UGGUgUCGugCCAGAGg -5' |
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| 9488 | 3' | -56.2 | NC_002531.1 | + | 10184 | 0.66 | 0.835743 |
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Target: 5'- uUGCACCAGCgGCCcguuaaaagugGCAGCaacuuguugACGGguuucuagaUCUCCa -3' miRNA: 3'- -AUGUGGUCG-UGG-----------UGUCG---------UGCC---------AGAGG- -5' |
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| 9488 | 3' | -56.2 | NC_002531.1 | + | 10085 | 0.72 | 0.4986 |
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Target: 5'- uUGCAcCCAGCAagGCAGCACacauccucugaGGUCUCUa -3' miRNA: 3'- -AUGU-GGUCGUggUGUCGUG-----------CCAGAGG- -5' |
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| 9488 | 3' | -56.2 | NC_002531.1 | + | 4149 | 0.67 | 0.818584 |
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Target: 5'- -uUAUCAGC-CaGCAGCugGGUUUCUg -3' miRNA: 3'- auGUGGUCGuGgUGUCGugCCAGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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