Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9489 | 5' | -54.3 | NC_002531.1 | + | 255 | 0.66 | 0.924349 |
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Target: 5'- cUUUCuuGCUGUgUGUCUgUGAGgagccUCGCa -3' miRNA: 3'- uAAAGu-CGACGgACAGGgACUC-----AGCG- -5' |
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| 9489 | 5' | -54.3 | NC_002531.1 | + | 37958 | 0.66 | 0.912569 |
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Target: 5'- ----aAGCUGaCagaGUCCCUGGGUgCGCu -3' miRNA: 3'- uaaagUCGAC-Gga-CAGGGACUCA-GCG- -5' |
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| 9489 | 5' | -54.3 | NC_002531.1 | + | 57266 | 0.67 | 0.888813 |
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Target: 5'- ---gCAGCUGaaauCUaGUCCCucuccggcaggugggUGAGUCGCg -3' miRNA: 3'- uaaaGUCGACg---GA-CAGGG---------------ACUCAGCG- -5' |
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| 9489 | 5' | -54.3 | NC_002531.1 | + | 92990 | 0.67 | 0.878715 |
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Target: 5'- -gUUUGGCUGCCagGggUCUGGGUUGCu -3' miRNA: 3'- uaAAGUCGACGGa-CagGGACUCAGCG- -5' |
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| 9489 | 5' | -54.3 | NC_002531.1 | + | 76592 | 0.7 | 0.73545 |
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Target: 5'- ---aUAGCUGCCUuugCCCUGGGcUGCu -3' miRNA: 3'- uaaaGUCGACGGAca-GGGACUCaGCG- -5' |
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| 9489 | 5' | -54.3 | NC_002531.1 | + | 57125 | 1.09 | 0.00285 |
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Target: 5'- gAUUUCAGCUGCCUGUCCCUGAGUCGCc -3' miRNA: 3'- -UAAAGUCGACGGACAGGGACUCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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