Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9490 | 5' | -53.1 | NC_002531.1 | + | 129388 | 0.66 | 0.955972 |
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Target: 5'- --aACGAAGC-CACCauGcccuacacccuuauGGGACCCCUGu -3' miRNA: 3'- aacUGCUUUGaGUGG--U--------------UCCUGGGGAC- -5' |
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| 9490 | 5' | -53.1 | NC_002531.1 | + | 116773 | 0.67 | 0.934461 |
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Target: 5'- -gGAgGGGACUUcgCAGGGACCCCc- -3' miRNA: 3'- aaCUgCUUUGAGugGUUCCUGGGGac -5' |
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| 9490 | 5' | -53.1 | NC_002531.1 | + | 24348 | 0.68 | 0.869871 |
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Target: 5'- aUGAgGAAAUUCuGCCAcGGGCCCUa- -3' miRNA: 3'- aACUgCUUUGAG-UGGUuCCUGGGGac -5' |
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| 9490 | 5' | -53.1 | NC_002531.1 | + | 62576 | 0.69 | 0.862063 |
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Target: 5'- gUGACGAuuGCUgGCCAGGuuGCCCCa- -3' miRNA: 3'- aACUGCUu-UGAgUGGUUCc-UGGGGac -5' |
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| 9490 | 5' | -53.1 | NC_002531.1 | + | 123940 | 0.7 | 0.801524 |
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Target: 5'- -gGACGGAgggguaaaACUgACCAAGGGCCaCCa- -3' miRNA: 3'- aaCUGCUU--------UGAgUGGUUCCUGG-GGac -5' |
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| 9490 | 5' | -53.1 | NC_002531.1 | + | 129433 | 0.75 | 0.532774 |
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Target: 5'- -gGugGGGACUUGCCuuuGGGCCCCUu -3' miRNA: 3'- aaCugCUUUGAGUGGuu-CCUGGGGAc -5' |
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| 9490 | 5' | -53.1 | NC_002531.1 | + | 129517 | 0.75 | 0.532774 |
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Target: 5'- -gGugGGGACUUGCCuuuGGGCCCCUu -3' miRNA: 3'- aaCugCUUUGAGUGGuu-CCUGGGGAc -5' |
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| 9490 | 5' | -53.1 | NC_002531.1 | + | 57247 | 1.02 | 0.010979 |
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Target: 5'- cUUGACGAAACUCACCAAGGACCCCUa -3' miRNA: 3'- -AACUGCUUUGAGUGGUUCCUGGGGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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