Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9491 | 3' | -55.1 | NC_002531.1 | + | 89461 | 0.68 | 0.793909 |
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Target: 5'- uGCcg-GGGGAGagaccucUGAGGCCugCCUg -3' miRNA: 3'- -CGuuaCCCCUCga-----ACUCUGGugGGAg -5' |
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| 9491 | 3' | -55.1 | NC_002531.1 | + | 21699 | 0.69 | 0.775066 |
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Target: 5'- aGCAAUcauuaggcaGGGGAGCaugccaGAGGCCACUC-Ca -3' miRNA: 3'- -CGUUA---------CCCCUCGaa----CUCUGGUGGGaG- -5' |
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| 9491 | 3' | -55.1 | NC_002531.1 | + | 93435 | 0.7 | 0.725785 |
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Target: 5'- gGCAuUGGGGAGCUgguaAGGCCcggucgguagGCCCa- -3' miRNA: 3'- -CGUuACCCCUCGAac--UCUGG----------UGGGag -5' |
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| 9491 | 3' | -55.1 | NC_002531.1 | + | 62990 | 0.7 | 0.692023 |
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Target: 5'- cGCAGUGGGGGGCgugccacaguuugcaGAGuaGCC-CCCUUu -3' miRNA: 3'- -CGUUACCCCUCGaa-------------CUC--UGGuGGGAG- -5' |
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| 9491 | 3' | -55.1 | NC_002531.1 | + | 121852 | 0.75 | 0.405407 |
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Target: 5'- gGCuGUGGGGAGUUUGGGuucuUCAUCCUUa -3' miRNA: 3'- -CGuUACCCCUCGAACUCu---GGUGGGAG- -5' |
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| 9491 | 3' | -55.1 | NC_002531.1 | + | 58289 | 1.12 | 0.001644 |
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Target: 5'- uGCAAUGGGGAGCUUGAGACCACCCUCa -3' miRNA: 3'- -CGUUACCCCUCGAACUCUGGUGGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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