Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9493 | 3' | -46.8 | NC_002531.1 | + | 122378 | 0.66 | 0.999795 |
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Target: 5'- -cGGGGACga--GAGCUUUUUCAGuGu -3' miRNA: 3'- guCUCCUGaaacUUCGGAAAAGUCuC- -5' |
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| 9493 | 3' | -46.8 | NC_002531.1 | + | 48504 | 0.66 | 0.999663 |
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Target: 5'- uCAGAGGuCUcUUGAGGCCU--UCAa-- -3' miRNA: 3'- -GUCUCCuGA-AACUUCGGAaaAGUcuc -5' |
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| 9493 | 3' | -46.8 | NC_002531.1 | + | 61230 | 0.69 | 0.99465 |
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Target: 5'- uGGAGGAgUcUGggGCCUgUUUgguGGAGg -3' miRNA: 3'- gUCUCCUgAaACuuCGGAaAAG---UCUC- -5' |
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| 9493 | 3' | -46.8 | NC_002531.1 | + | 116734 | 0.69 | 0.993746 |
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Target: 5'- uGGAGGAUUUUGAGGaCUUaggUCuGGAGg -3' miRNA: 3'- gUCUCCUGAAACUUCgGAAa--AG-UCUC- -5' |
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| 9493 | 3' | -46.8 | NC_002531.1 | + | 9891 | 0.69 | 0.993649 |
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Target: 5'- cCAGAGGACaccaaagcgGucGCCUUUagagaccUCAGAGg -3' miRNA: 3'- -GUCUCCUGaaa------CuuCGGAAA-------AGUCUC- -5' |
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| 9493 | 3' | -46.8 | NC_002531.1 | + | 5824 | 0.7 | 0.990814 |
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Target: 5'- aAGAGGACUUcaauucuacagaauaUGAuGUCUUUUUAGAc -3' miRNA: 3'- gUCUCCUGAA---------------ACUuCGGAAAAGUCUc -5' |
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| 9493 | 3' | -46.8 | NC_002531.1 | + | 9095 | 0.7 | 0.987066 |
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Target: 5'- -cGAGGACga-GGAGCCUguauuuccccucuUUUCAGAa -3' miRNA: 3'- guCUCCUGaaaCUUCGGA-------------AAAGUCUc -5' |
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| 9493 | 3' | -46.8 | NC_002531.1 | + | 61041 | 1.11 | 0.013686 |
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Target: 5'- gCAGAGGACUUUGAAGCCUUUUCAGAGg -3' miRNA: 3'- -GUCUCCUGAAACUUCGGAAAAGUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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