Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9497 | 3' | -60.5 | NC_002531.1 | + | 12571 | 0.67 | 0.584253 |
Target: 5'- gAGGCUGGagGCGGgggcgccgguaaagGCCGGGGA-CCCa -3' miRNA: 3'- gUCUGACC--CGCCa-------------UGGCCCUUcGGGa -5' |
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9497 | 3' | -60.5 | NC_002531.1 | + | 70443 | 1.06 | 0.001441 |
Target: 5'- gCAGACUGGGCGGUACCGGGAAGCCCUu -3' miRNA: 3'- -GUCUGACCCGCCAUGGCCCUUCGGGA- -5' |
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9497 | 3' | -60.5 | NC_002531.1 | + | 103284 | 0.7 | 0.429035 |
Target: 5'- gGGAUgaauggagGGcGUGGUGCUacgaGGGAGGCCCUa -3' miRNA: 3'- gUCUGa-------CC-CGCCAUGG----CCCUUCGGGA- -5' |
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9497 | 3' | -60.5 | NC_002531.1 | + | 130182 | 0.67 | 0.580263 |
Target: 5'- -uGACgGGGCGG--CCGGGGgaGGUCCg -3' miRNA: 3'- guCUGaCCCGCCauGGCCCU--UCGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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