Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9497 | 5' | -54.6 | NC_002531.1 | + | 55918 | 0.66 | 0.893076 |
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Target: 5'- ---uGG-CUGCUUuUUGGCCUGUCCc -3' miRNA: 3'- auuuCCgGACGGGuAAUCGGACAGGu -5' |
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| 9497 | 5' | -54.6 | NC_002531.1 | + | 55646 | 0.66 | 0.878669 |
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Target: 5'- --cAGGCU--CCCGUUGGCCauccaggGUCCAa -3' miRNA: 3'- auuUCCGGacGGGUAAUCGGa------CAGGU- -5' |
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| 9497 | 5' | -54.6 | NC_002531.1 | + | 109811 | 0.71 | 0.649747 |
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Target: 5'- aUAGAGGCCcuggagGCCCGUUaggaccagGGCCUagguggcccgGUCCAg -3' miRNA: 3'- -AUUUCCGGa-----CGGGUAA--------UCGGA----------CAGGU- -5' |
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| 9497 | 5' | -54.6 | NC_002531.1 | + | 103858 | 0.74 | 0.453121 |
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Target: 5'- uUGAGGGCCUGCCCcggGGUUUG-CCGg -3' miRNA: 3'- -AUUUCCGGACGGGuaaUCGGACaGGU- -5' |
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| 9497 | 5' | -54.6 | NC_002531.1 | + | 19000 | 0.77 | 0.307886 |
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Target: 5'- aAAAGGUUUGCCCAgaacCCUGUCCAg -3' miRNA: 3'- aUUUCCGGACGGGUaaucGGACAGGU- -5' |
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| 9497 | 5' | -54.6 | NC_002531.1 | + | 70478 | 1.06 | 0.003492 |
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Target: 5'- cUAAAGGCCUGCCCAUUAGCCUGUCCAc -3' miRNA: 3'- -AUUUCCGGACGGGUAAUCGGACAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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