Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9499 | 5' | -44.8 | NC_002531.1 | + | 14362 | 0.66 | 0.999983 |
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Target: 5'- gCUGUucCCUGgUGAguguagcuagGCUUUAGCAGGu-- -3' miRNA: 3'- -GAUA--GGACgACU----------UGAAAUCGUCUuug -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 15011 | 0.66 | 0.999976 |
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Target: 5'- -aAUUUUGCUGGAgUaaagauGCAGAAACa -3' miRNA: 3'- gaUAGGACGACUUgAaau---CGUCUUUG- -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 6459 | 0.66 | 0.999967 |
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Target: 5'- -cAUCUUGCUGca-UUUAGCAaAAGCa -3' miRNA: 3'- gaUAGGACGACuugAAAUCGUcUUUG- -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 98346 | 0.67 | 0.999918 |
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Target: 5'- gCUGcCCUGgaGAGCU-UGGCAGucauGAGCa -3' miRNA: 3'- -GAUaGGACgaCUUGAaAUCGUC----UUUG- -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 64837 | 0.67 | 0.999918 |
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Target: 5'- cCUGUCCagauaCUGGAUg--AGUAGAGACg -3' miRNA: 3'- -GAUAGGac---GACUUGaaaUCGUCUUUG- -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 76474 | 0.68 | 0.999811 |
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Target: 5'- aCUGUg--GCUGGGCUcugagaugUUGGCAGAAAUg -3' miRNA: 3'- -GAUAggaCGACUUGA--------AAUCGUCUUUG- -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 9988 | 0.73 | 0.978357 |
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Target: 5'- --cUCCUGCUGGcaUUUGGUAGAAGa -3' miRNA: 3'- gauAGGACGACUugAAAUCGUCUUUg -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 59812 | 0.77 | 0.916653 |
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Target: 5'- aUcgCCUGCUGAACUUcugGGCAGcGGAUa -3' miRNA: 3'- gAuaGGACGACUUGAAa--UCGUC-UUUG- -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 99209 | 0.78 | 0.872987 |
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Target: 5'- --uUCCUGUUGGACUaUAGCGGAcaggGACu -3' miRNA: 3'- gauAGGACGACUUGAaAUCGUCU----UUG- -5' |
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| 9499 | 5' | -44.8 | NC_002531.1 | + | 78103 | 1.12 | 0.018473 |
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Target: 5'- cCUAUCCUGCUGAACUUUAGCAGAAACu -3' miRNA: 3'- -GAUAGGACGACUUGAAAUCGUCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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