Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
95 | 3' | -64.7 | AC_000006.1 | + | 25172 | 0.66 | 0.227557 |
Target: 5'- cUCGCGGCucagguacuccgaGGUCGUGcaGCCCgGCcuGCAg -3' miRNA: 3'- -GGCGCCGug-----------CCAGCAC--CGGGgCG--CGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 10775 | 0.66 | 0.219641 |
Target: 5'- cCCGCGuGCAgcugcagaaGGaCGUGcGCCCgGCGUAc -3' miRNA: 3'- -GGCGC-CGUg--------CCaGCAC-CGGGgCGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 28845 | 0.66 | 0.219641 |
Target: 5'- aCCGCGGCugGcGcgucaggaUCGU-GCCCgacgaGCGCAc -3' miRNA: 3'- -GGCGCCGugC-C--------AGCAcCGGGg----CGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 25483 | 0.66 | 0.214131 |
Target: 5'- cCUGgGGCAUGGcCGagcGGCUCCGCauccaGCAa -3' miRNA: 3'- -GGCgCCGUGCCaGCa--CCGGGGCG-----CGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 22673 | 0.66 | 0.19831 |
Target: 5'- cCCGUGGCccCGGggcggaGUGGCCUCuCGCu -3' miRNA: 3'- -GGCGCCGu-GCCag----CACCGGGGcGCGu -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 17125 | 0.66 | 0.19831 |
Target: 5'- uCCGUGGuCACaucgugGGUCGgGGCCaCCGUGgGg -3' miRNA: 3'- -GGCGCC-GUG------CCAGCaCCGG-GGCGCgU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 22852 | 0.67 | 0.188337 |
Target: 5'- gCGCGGCACGuUCGccGGCUgCGuCGCc -3' miRNA: 3'- gGCGCCGUGCcAGCa-CCGGgGC-GCGu -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 5250 | 0.67 | 0.18785 |
Target: 5'- cUCGUaguugagGGCcucgGCGG-CGUGGCCCUugGCGCGg -3' miRNA: 3'- -GGCG-------CCG----UGCCaGCACCGGGG--CGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 6215 | 0.67 | 0.186396 |
Target: 5'- gCCGCGGUuaugcagggugaccaGGUCcacgcugGUGGCCaccucgCCGCGCAg -3' miRNA: 3'- -GGCGCCGug-------------CCAG-------CACCGG------GGCGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 21739 | 0.67 | 0.174206 |
Target: 5'- cCCGCuGC-CGGUCGUGGCgCUGUc-- -3' miRNA: 3'- -GGCGcCGuGCCAGCACCGgGGCGcgu -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 14096 | 0.68 | 0.161027 |
Target: 5'- uCCGCaGGCgGCGGagUCG-GGCCCC-UGCAc -3' miRNA: 3'- -GGCG-CCG-UGCC--AGCaCCGGGGcGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 8364 | 0.68 | 0.161027 |
Target: 5'- -gGCGGCAgaggcacguCGG-CGUGGCgCUCGgGCAg -3' miRNA: 3'- ggCGCCGU---------GCCaGCACCG-GGGCgCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 17021 | 0.69 | 0.133714 |
Target: 5'- uCCGCGGCACGGcaCGcGGCCguucaUGgGCAc -3' miRNA: 3'- -GGCGCCGUGCCa-GCaCCGGg----GCgCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 17273 | 0.69 | 0.13018 |
Target: 5'- aCUGCGGCAUcaucgaGGggGUGGCCaucuuCGCGCGu -3' miRNA: 3'- -GGCGCCGUG------CCagCACCGGg----GCGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 11079 | 0.69 | 0.120087 |
Target: 5'- aUCGCGcGCGaGGagGUGGCCCUGgGCc -3' miRNA: 3'- -GGCGC-CGUgCCagCACCGGGGCgCGu -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 19579 | 0.7 | 0.113768 |
Target: 5'- gCGCGGCgcGCGGcUCGcaucaUGGCCCgGCGg- -3' miRNA: 3'- gGCGCCG--UGCC-AGC-----ACCGGGgCGCgu -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 10152 | 0.7 | 0.099306 |
Target: 5'- -gGuCGGCACGGUC-UGGCCggugagaCGCGCGc -3' miRNA: 3'- ggC-GCCGUGCCAGcACCGGg------GCGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 22290 | 0.71 | 0.094021 |
Target: 5'- gCGCGGCGCuGGgaGUGGCCCCcuccUGCAu -3' miRNA: 3'- gGCGCCGUG-CCagCACCGGGGc---GCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 24142 | 0.71 | 0.089004 |
Target: 5'- aCGCGcugGCGGUCcaGGCCCuCGCGCAg -3' miRNA: 3'- gGCGCcg-UGCCAGcaCCGGG-GCGCGU- -5' |
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95 | 3' | -64.7 | AC_000006.1 | + | 21763 | 0.72 | 0.071371 |
Target: 5'- gCCGCGGCGCGGgCGcaggcucccuaUGGUCCUgaGCGUAg -3' miRNA: 3'- -GGCGCCGUGCCaGC-----------ACCGGGG--CGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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