Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9501 | 3' | -51.6 | NC_002531.1 | + | 25011 | 0.66 | 0.971818 |
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Target: 5'- uGUCCuucagGC-GCUUCAcCACCACCAa -3' miRNA: 3'- uUAGGua---CGuUGGAGUuGUGGUGGUa -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 80944 | 0.66 | 0.968704 |
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Target: 5'- --cCCGUGC-ACCUCAACcAgCACCc- -3' miRNA: 3'- uuaGGUACGuUGGAGUUG-UgGUGGua -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 15456 | 0.66 | 0.956709 |
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Target: 5'- uGUCC-UGCAagagucacuggcuuACCUCAaaGCACaCACCAUc -3' miRNA: 3'- uUAGGuACGU--------------UGGAGU--UGUG-GUGGUA- -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 13122 | 0.68 | 0.911323 |
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Target: 5'- ------aGCAGCUUCAGCAuCCACCAg -3' miRNA: 3'- uuagguaCGUUGGAGUUGU-GGUGGUa -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 80687 | 0.69 | 0.875215 |
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Target: 5'- -cUCCGgccugGCAgccaaggGCCUCAGCAUCACCc- -3' miRNA: 3'- uuAGGUa----CGU-------UGGAGUUGUGGUGGua -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 103730 | 0.69 | 0.860068 |
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Target: 5'- -uUCUaauGUGCAggcGCCUcCGGCGCCGCCAc -3' miRNA: 3'- uuAGG---UACGU---UGGA-GUUGUGGUGGUa -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 84902 | 0.71 | 0.806913 |
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Target: 5'- uAUCCGUGCuguuuACC-CAGCACCACg-- -3' miRNA: 3'- uUAGGUACGu----UGGaGUUGUGGUGgua -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 60236 | 0.71 | 0.79736 |
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Target: 5'- --aCCGUGgAauucACCUCGACuACCACCAUu -3' miRNA: 3'- uuaGGUACgU----UGGAGUUG-UGGUGGUA- -5' |
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| 9501 | 3' | -51.6 | NC_002531.1 | + | 82589 | 1.04 | 0.009166 |
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Target: 5'- gAAUCCAUGCAACCUCAACACCACCAUg -3' miRNA: 3'- -UUAGGUACGUUGGAGUUGUGGUGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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