Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9504 | 3' | -51.7 | NC_002531.1 | + | 56093 | 0.66 | 0.976097 |
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Target: 5'- cUUUCCugGGaagaaUGUAUGCAGGGUGu- -3' miRNA: 3'- uGAAGGugUCg----ACGUACGUUCUACcc -5' |
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| 9504 | 3' | -51.7 | NC_002531.1 | + | 47597 | 0.66 | 0.976097 |
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Target: 5'- gGCUUCCACua-UGUA-GCAgaugaauggauAGGUGGGg -3' miRNA: 3'- -UGAAGGUGucgACGUaCGU-----------UCUACCC- -5' |
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| 9504 | 3' | -51.7 | NC_002531.1 | + | 7622 | 0.68 | 0.93831 |
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Target: 5'- --cUCCAUGGCUGCAU-CGAGcgucucagcuGUGGGu -3' miRNA: 3'- ugaAGGUGUCGACGUAcGUUC----------UACCC- -5' |
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| 9504 | 3' | -51.7 | NC_002531.1 | + | 16485 | 0.68 | 0.933145 |
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Target: 5'- aGCUUCUAaacaaugggcUAGCUGCAcguuggGCGGGcUGGGa -3' miRNA: 3'- -UGAAGGU----------GUCGACGUa-----CGUUCuACCC- -5' |
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| 9504 | 3' | -51.7 | NC_002531.1 | + | 65495 | 0.71 | 0.797425 |
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Target: 5'- uGCUcCCACAGCU-CAUGCAAGucauuuUGGa -3' miRNA: 3'- -UGAaGGUGUCGAcGUACGUUCu-----ACCc -5' |
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| 9504 | 3' | -51.7 | NC_002531.1 | + | 90248 | 1.1 | 0.004683 |
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Target: 5'- cACUUCCACAGCUGCAUGCAAGAUGGGc -3' miRNA: 3'- -UGAAGGUGUCGACGUACGUUCUACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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