Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9504 | 5' | -58.5 | NC_002531.1 | + | 24924 | 0.66 | 0.728362 |
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Target: 5'- aGGCCaacUGCCACUgguucacaggugCGCAGCUucacUGGCUg -3' miRNA: 3'- -UCGGgu-GCGGUGA------------GUGUCGA----ACCGAa -5' |
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| 9504 | 5' | -58.5 | NC_002531.1 | + | 77275 | 0.66 | 0.70813 |
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Target: 5'- cGUCUACuCCACUCugAGCUaccUGGUg- -3' miRNA: 3'- uCGGGUGcGGUGAGugUCGA---ACCGaa -5' |
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| 9504 | 5' | -58.5 | NC_002531.1 | + | 122450 | 0.66 | 0.70813 |
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Target: 5'- aGGCCUcCGUCGCUCACAuCgUGGCc- -3' miRNA: 3'- -UCGGGuGCGGUGAGUGUcGaACCGaa -5' |
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| 9504 | 5' | -58.5 | NC_002531.1 | + | 57812 | 0.67 | 0.635702 |
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Target: 5'- -aCCCACuGCCGCUCAC-GCUgGGUa- -3' miRNA: 3'- ucGGGUG-CGGUGAGUGuCGAaCCGaa -5' |
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| 9504 | 5' | -58.5 | NC_002531.1 | + | 50260 | 0.68 | 0.563087 |
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Target: 5'- gAGCCCaACGCgGCUCGCA---UGGCa- -3' miRNA: 3'- -UCGGG-UGCGgUGAGUGUcgaACCGaa -5' |
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| 9504 | 5' | -58.5 | NC_002531.1 | + | 35641 | 0.69 | 0.542683 |
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Target: 5'- gAGgCCGgG-CGCUCGCAGCUgUGGCUg -3' miRNA: 3'- -UCgGGUgCgGUGAGUGUCGA-ACCGAa -5' |
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| 9504 | 5' | -58.5 | NC_002531.1 | + | 80674 | 0.7 | 0.463997 |
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Target: 5'- uGGUCCugGgCCGCUC-CGGCcUGGCa- -3' miRNA: 3'- -UCGGGugC-GGUGAGuGUCGaACCGaa -5' |
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| 9504 | 5' | -58.5 | NC_002531.1 | + | 90282 | 1.04 | 0.002088 |
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Target: 5'- aAGCCCACGCCACUCACAGCUUGGCUUu -3' miRNA: 3'- -UCGGGUGCGGUGAGUGUCGAACCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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