Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9505 | 5' | -51.4 | NC_002531.1 | + | 24306 | 0.66 | 0.977146 |
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Target: 5'- aCAGCCucUGCCCaACCAucGUCUcUgGUGg -3' miRNA: 3'- -GUCGGuuACGGG-UGGU--CAGAaAgUAU- -5' |
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| 9505 | 5' | -51.4 | NC_002531.1 | + | 107547 | 0.66 | 0.96512 |
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Target: 5'- -uGCgAGUGCCUgGCCAGUCaUUCAg- -3' miRNA: 3'- guCGgUUACGGG-UGGUCAGaAAGUau -5' |
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| 9505 | 5' | -51.4 | NC_002531.1 | + | 14477 | 0.67 | 0.957681 |
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Target: 5'- aCAGCCA--GCCC-CCAGgCUUUUAa- -3' miRNA: 3'- -GUCGGUuaCGGGuGGUCaGAAAGUau -5' |
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| 9505 | 5' | -51.4 | NC_002531.1 | + | 8776 | 0.67 | 0.953582 |
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Target: 5'- aCAGCCAAUGCCC-CCuguGUggUUCc-- -3' miRNA: 3'- -GUCGGUUACGGGuGGu--CAgaAAGuau -5' |
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| 9505 | 5' | -51.4 | NC_002531.1 | + | 60272 | 0.67 | 0.944603 |
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Target: 5'- aCAGCCGAUGCCCuccucaacuACCAGcUCa------ -3' miRNA: 3'- -GUCGGUUACGGG---------UGGUC-AGaaaguau -5' |
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| 9505 | 5' | -51.4 | NC_002531.1 | + | 11663 | 0.71 | 0.807297 |
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Target: 5'- uCAGaCAucUGCCCACCGGUCUggCAa- -3' miRNA: 3'- -GUCgGUu-ACGGGUGGUCAGAaaGUau -5' |
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| 9505 | 5' | -51.4 | NC_002531.1 | + | 111793 | 0.76 | 0.554223 |
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Target: 5'- uGGCCcuUGCCCACCGGg--UUCAUAc -3' miRNA: 3'- gUCGGuuACGGGUGGUCagaAAGUAU- -5' |
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| 9505 | 5' | -51.4 | NC_002531.1 | + | 90599 | 1.07 | 0.006786 |
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Target: 5'- aCAGCCAAUGCCCACCAGUCUUUCAUAc -3' miRNA: 3'- -GUCGGUUACGGGUGGUCAGAAAGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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