Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9506 | 3' | -54.9 | NC_002531.1 | + | 48009 | 0.66 | 0.872491 |
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Target: 5'- cUACUAcuAUGUGAuGCUGCCgGuGUGCACc -3' miRNA: 3'- -AUGGUu-UACACU-CGGUGGgC-CACGUG- -5' |
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| 9506 | 3' | -54.9 | NC_002531.1 | + | 35051 | 0.67 | 0.856897 |
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Target: 5'- cUGCCuuugggGAGUGuUGGGCCA--CGGUGCACu -3' miRNA: 3'- -AUGG------UUUAC-ACUCGGUggGCCACGUG- -5' |
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| 9506 | 3' | -54.9 | NC_002531.1 | + | 20409 | 0.67 | 0.84877 |
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Target: 5'- -uCCAGgugcGUGUGGGCauCuuGGUGCACg -3' miRNA: 3'- auGGUU----UACACUCGguGggCCACGUG- -5' |
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| 9506 | 3' | -54.9 | NC_002531.1 | + | 22550 | 0.68 | 0.805129 |
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Target: 5'- cUGCaGAAUGUuGGCCACCCcGUGUAUg -3' miRNA: 3'- -AUGgUUUACAcUCGGUGGGcCACGUG- -5' |
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| 9506 | 3' | -54.9 | NC_002531.1 | + | 117716 | 0.72 | 0.568436 |
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Target: 5'- --aCGAGUGUGAGCCACauaCGccuucuaGUGCACg -3' miRNA: 3'- augGUUUACACUCGGUGg--GC-------CACGUG- -5' |
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| 9506 | 3' | -54.9 | NC_002531.1 | + | 92238 | 1.09 | 0.002491 |
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Target: 5'- aUACCAAAUGUGAGCCACCCGGUGCACa -3' miRNA: 3'- -AUGGUUUACACUCGGUGGGCCACGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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