Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
9507 | 3' | -50 | NC_002531.1 | + | 102287 | 1.09 | 0.007499 |
Target: 5'- aGCAGAAGCGACAGUGAAGACUCAGAAg -3' miRNA: 3'- -CGUCUUCGCUGUCACUUCUGAGUCUU- -5' |
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9507 | 3' | -50 | NC_002531.1 | + | 80361 | 0.71 | 0.909103 |
Target: 5'- --cGAGGUGACGGUGAAGACcgaGGGc -3' miRNA: 3'- cguCUUCGCUGUCACUUCUGag-UCUu -5' |
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9507 | 3' | -50 | NC_002531.1 | + | 100478 | 0.71 | 0.909103 |
Target: 5'- uGguGGAGCGAgCAGUGAGGGC-CAu-- -3' miRNA: 3'- -CguCUUCGCU-GUCACUUCUGaGUcuu -5' |
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9507 | 3' | -50 | NC_002531.1 | + | 96404 | 0.7 | 0.93809 |
Target: 5'- uGguGGAGCGAUGGguuggGuuGAUUCAGAAa -3' miRNA: 3'- -CguCUUCGCUGUCa----CuuCUGAGUCUU- -5' |
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9507 | 3' | -50 | NC_002531.1 | + | 12562 | 0.68 | 0.976354 |
Target: 5'- gGCGGggGCGcCGGUaAAGGC-CGGGGa -3' miRNA: 3'- -CGUCuuCGCuGUCAcUUCUGaGUCUU- -5' |
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9507 | 3' | -50 | NC_002531.1 | + | 99194 | 0.67 | 0.983322 |
Target: 5'- aGCGGAcAGgGACuGUGAgggGGGCUCuGGAg -3' miRNA: 3'- -CGUCU-UCgCUGuCACU---UCUGAGuCUU- -5' |
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9507 | 3' | -50 | NC_002531.1 | + | 111251 | 0.67 | 0.985259 |
Target: 5'- uGUGGAaaacaGGC-ACAGaGAGGACUCAGAc -3' miRNA: 3'- -CGUCU-----UCGcUGUCaCUUCUGAGUCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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