Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 9509 | 5' | -55 | NC_002531.1 | + | 87393 | 0.65 | 0.908282 |
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Target: 5'- -----cCGCCCAGGCCgAGCAuuUCa- -3' miRNA: 3'- accuaaGUGGGUCCGGgUCGUu-AGac -5' |
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| 9509 | 5' | -55 | NC_002531.1 | + | 34949 | 0.66 | 0.901874 |
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Target: 5'- cGGGUUCAUCUuuuGuGCCUAGCAGgUCg- -3' miRNA: 3'- aCCUAAGUGGGu--C-CGGGUCGUU-AGac -5' |
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| 9509 | 5' | -55 | NC_002531.1 | + | 98067 | 0.66 | 0.888317 |
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Target: 5'- gGGAcUCuCCUGGGCCCAGagggaaacgccCAGUUUGa -3' miRNA: 3'- aCCUaAGuGGGUCCGGGUC-----------GUUAGAC- -5' |
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| 9509 | 5' | -55 | NC_002531.1 | + | 17630 | 0.69 | 0.749779 |
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Target: 5'- -aGAguaCACCCucucauGGGCCCAGCAAUUUa -3' miRNA: 3'- acCUaa-GUGGG------UCCGGGUCGUUAGAc -5' |
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| 9509 | 5' | -55 | NC_002531.1 | + | 35217 | 0.69 | 0.748784 |
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Target: 5'- cUGGAgugCACCguGGCCCAacacuccccaaagGCAgAUCUGc -3' miRNA: 3'- -ACCUaa-GUGGguCCGGGU-------------CGU-UAGAC- -5' |
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| 9509 | 5' | -55 | NC_002531.1 | + | 20090 | 0.69 | 0.72969 |
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Target: 5'- uUGGA-UCAUCCucGGCCaugcGCAGUCUGg -3' miRNA: 3'- -ACCUaAGUGGGu-CCGGgu--CGUUAGAC- -5' |
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| 9509 | 5' | -55 | NC_002531.1 | + | 74246 | 0.7 | 0.678073 |
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Target: 5'- aUGGGU--AUCCAGGCCCAGUcacuAAUCa- -3' miRNA: 3'- -ACCUAagUGGGUCCGGGUCG----UUAGac -5' |
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| 9509 | 5' | -55 | NC_002531.1 | + | 108412 | 1.08 | 0.003165 |
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Target: 5'- cUGGAUUCACCCAGGCCCAGCAAUCUGc -3' miRNA: 3'- -ACCUAAGUGGGUCCGGGUCGUUAGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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