miRNA: 3'- -UCAAUAaaCGGcGGGuACGGGG-GGUC- -5'
miRNA: 3'- ucaauaaACgGCGGGUACGGGGGGuC- -5'
miRNA: 3'- -UCAAUAAACGGCGGGUACGGGGGGUC- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 953 | 5' | -56.1 | NC_000902.1 | + | 31751 | 0.66 | 0.662052 |
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Target: 5'- gGGUUGUg-GCC-CCC-UGCUCCaCCAGg -3' miRNA: 3'- -UCAAUAaaCGGcGGGuACGGGG-GGUC- -5' |
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| 953 | 5' | -56.1 | NC_000902.1 | + | 15993 | 0.75 | 0.210037 |
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Target: 5'- ------aUGgUGCCCAUGCCCCCCuGg -3' miRNA: 3'- ucaauaaACgGCGGGUACGGGGGGuC- -5' |
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| 953 | 5' | -56.1 | NC_000902.1 | + | 19831 | 1.08 | 0.001015 |
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Target: 5'- uAGUUAUUUGCCGCCCAUGCCCCCCAGc -3' miRNA: 3'- -UCAAUAAACGGCGGGUACGGGGGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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